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《Qilu Pharmaceutical Affairs》 2009-01
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Determination of puerarin in Shiwei Jiangtang Capsule by HPLC

DING Ai-ying,LI Yan-ru,QU Jia,KANG Ying-ying,Zang You-jia(Jilin Yimintang Pharmaceutical Co.,Ltd.,Siping 136001)  
OBJECTIVETo determine the contents of the puerarin in Shiwei Jiangtang Capsule by HPLC.METHODSRP-HPLC was used with a column of Agilent XDB C18(250mm×4.6mm,5μm) and a mobile phase of methanol-phosphate buffer(20∶80),the flow rate was 1mL·min-1,the detection wavelength was 250nm and the column temperature was 25℃,the injection volume was 10μL.RESULTSThe retention time of the puerarin was about 27min,the resolution factor between puerarin and adjacent peak is not less than 2.0.Good linear correlation existed,when the puerarin was within 0.024~0.476μg.The correlation coefficient was 0.9999.The average recoveries of paeoniflorin were 98.81%(n=5).CONCLUSIONThis method was accurate,reliable and can be used to control puerarin in Shiwei Jiangtang Capsule.
【CateGory Index】: R286.0
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【References】
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1 DU Wei-qi1,SHI Xiu-fang2,ZHANG Wen-bo3,ZHANG Zhen-zhong2(1.Drug and Equipment Section,Anyang Regional Hospital,Anyang 455000,Henan Province,China;2.School of Pharmaceutical Science,Zhengzhou University,Zhengzhou 450001,Henan Province,China;3.Department of Life Science and Technology,Xinxiang Medical College,Xinxiang 453003,Henan Province,China);Determination of puerarin in Shiwei Jiangtang effervescent granules by high performance liquid chromatography[J];Journal of Xinxiang Medical College;2011-06
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1 LU Wei-kun,ZENG Fang,LI Yuan(Pharmaceutical Preparation Section,First Affiliated Hospital,Sun Yat-sen University,Guangzhou,Guangdong 510700,China);Content analysis of puerarin in Yiqijiangtang capsules by HPLC[J];Journal of Guangdong College of Pharmacy;2008-05
2 ;Advances in treatment of diabets mellitus with puerarin and mechanism of its complications[J];Journal of Chinese PLA Postgraduate Medical School;2009-06
3 YAN Su-qin,SONG Xiao-lin,LI Xiang-yong (Jiangsu Lianyungang Chinese Medical Hospital,Lianyungang 222004,China);Determination of Puerarin Content in Jiangtang Pill for Invigorating Qi Nourishing Yin by HPLC Method[J];Lishizhen Medicine and Materia Medica Research;2007-05
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【Secondary References】
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1 WANG Wenqiong Jingzhou Central Hospital,Hubei Province,Jingzhou 434020,China;Application of ultra performance liquid chromatography in pharmaceutical analysis area[J];China Medical Herald;2012-11
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