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《Qilu Pharmaceutical Affairs》 2009-01
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Study on the pharmacokinetics of talinolol tablets in healthy volunteers with HPLC-MS/MS assay

ZHOU Zheng-jun(The First People′s Hospital of Changde City,Changde 415000)  
OBJECTIVETo establish a rapid and sensitive HPLC-MS/MS method determination of talinolol in human plasma and study the pharmacokinetics of talinolol tablets in healthy volunteers.METHODSThe samples were prepared by protein precipitation method using acetonitrile.After centrifugalized the upper layer was removed into injection bottle and prepared to inject into HPLC system.The samples were analyzed by a reverse phase high-performance liquid chromatography-electrospray ionization tandem mass spectrometry(HPLC-ESI-MS/MS),with positive ion SIM detection of talinolol(parent ion:364.5,daughter ion:308.4) using fluoxetine hydrochloride(parent ion:310.1,daughter ion:148.3) as internal standard.The HPLC separation of the analytes was performed on a Phenomenex C18 pre-column(10mm×4.6mm,5μm) and Phenomenex C18 column(250mm×4.6mm,5μm),with a flow rate of 0.9mL·min-1.RESULTSThe complete resolution was obtained in less than 9min.The calibration curve was linear in 2.0~320.0ng·mL-1 for talinolol,with a corelation coeficient above 0.9976.The average extraction recovery was above 77%.The methodology recovery was above 91% and less than 103%.The limit of quantitation(LOQ) was 2.0ng·mL-1 for talinolol.The intra-day and inter-day relative standard deviations were less than 9%.A single 50mg dose of talinolol tablet was administered to 12 healthy Chinese volunteers,the main pharmaeokinetie data are as follows:Cmax(91.90±48.09)ng·mL-1;tmax(2.79±0.89) h;t(14.67±2.15)h.CONCLUSIONThe method was accurate,sensitive and simple for the pharmacokinetie study of talinolol.
【CateGory Index】: R969.1
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 HE Juan ZANG Cong SONG Juan TANG Jing LIU Xiao-lei PENG Wen-xing (Clinical Pharmacy Research Institute of Second Xiangya Hospital of Central South University,Changsha 410011,China);HPLC-MS determination of talinolol in human plasma and application to pharmacokinetic studies on Chinese healthy volunteers[J];Chinese Journal of Pharmaceutical Analysis;2007-11
【Co-citations】
Chinese Journal Full-text Database 4 Hits
1 HE Juan ZANG Cong SONG Juan TANG Jing LIU Xiao-lei PENG Wen-xing (Clinical Pharmacy Research Institute of Second Xiangya Hospital of Central South University,Changsha 410011,China);HPLC-MS determination of talinolol in human plasma and application to pharmacokinetic studies on Chinese healthy volunteers[J];Chinese Journal of Pharmaceutical Analysis;2007-11
2 HE Xin~(1,2) YANG Guo-ping~3 TAN Zhi-rong~1 CHEN Yao~1 GUO Dong~1 PENG Liang~1 CHEN Hao~1 OUYANG Dong-sheng~(1*) (1.Institute of Clinical Pharmacology,Central South University,Changsha 410078,China; 2.The Third Hospital of Changsha City,Changsha 410010,China; 3.The Third Xiangya Hospital of Central South University,Changsha 410013,China);HPLC determination of talinolol in human plasma[J];Chinese Journal of Pharmaceutical Analysis;2008-06
3 WANG Yi-nan,TAN Zhi-rong,CHEN Yao,GUO Dong,HAN Yang,TAO Gong-you,CHEN Hao,CHEN Ying,XIAO Chang-qiong,ZHANG-Min,ZHOU Hong-hao(Institute of Clinical Pharmacology,Central South University,Changsha 410078);Determination of the concentration of Talinolol in healthy volunteers by LC-MS[J];Central South Pharmacy;2008-01
4 Liu Bo~1,Peng Kelong~2(1.The Second Clinical Medical College,Tongji Medical College,Huazhong University of Science and Technology, Wuhan 430030,China;2.School of Pharmacy,Tongji Medical College of Huazhong University of Science and Technology);Determination of Ambroxol in Human Plasma by LC-MS/MS:Application in a Bioequivalence Study[J];China Pharmacist;2011-03
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