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《Journal of Shandong University of Traditional Chinese Medicine》 2010-02
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Study on effective fraction of anti-influenza virus of flos lonicerae japonicae in vitro

SHI Jun-ying,GUO Cheng-jun(Shandong University of Traditional Chinese Medicine,Jinan 250355,China)  
Objective:To screen effective fraction of anti-influenza virus(IV) extracted from flos lonicerae japonicae,which would provide evidence for explanation of active substance foundation of flos lonicerae japonicae.Methods:Through the protection of various part extracted from flos lonicerae japonicae to MDCK affected by IV A and B,percentage of cytopathic effect(CPE) was observed,EC50 and TI was determined by Reed-Muench method,and hoped to find its anti-IV active fraction.Results:There had exact anti-IV activity in the various extracts of petroleum ether,acetone,ethanol,water and crude extract from flos lonicerae japonicae,but the extracts of chloroform and acetic ether had little;in fact,EC50 of the extract of petroleum and ethanol were less than which of ribovirin.Conclusion:Flos lonicerae japonicae had exact anti-IV activity,the most active ingredients focus in the extracts of petroleum and ethanol,they achieved better effect than ribovirin.
【Fund】: 山东省自然科学基金资助项目(编号:2007ZRB14248)
【CateGory Index】: R285
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