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《Journal of Shanghai Jiaotong University》 2005-S1
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A Simple Kinetic Model for Myeloma Cell Growth with Lysine as a Limiting Substrate

ZHANG Fan1, BI Jing-xiu2, ZENG An-ping3, YUAN Jing-qi1(1. Dept. of Automation, Shanghai Jiaotong Univ., Shanghai 200030, China; 2. National Key Lab. of Biochemical Eng., Inst. of Process Eng., Chinese Academy of Science, Beijing 100080; 3. Division of Molecular Biotechnology, GBF-Gesellschaft fuer Biotechnologische Forschung GmbH, 38124-Braunschweig, Germany)  
The nutrient condition for mammalian cells in vitro such as amino acids and growth factors plays important role on cell growth. Lysine is found to be an important limiting amino acid in myeloma cell cultivation. Therefore, an unstructured kinetic model which takes the influence of lysine concentration into account was developed. In this model, intracellular lysine and extracellular lysine(lysine in culture medium) is differentiated, and the intracellular lysine is assumed to affect the cell growth directly. The simulation shows that the model is able to describe the cell growth under changing culture conditions.
【Fund】: 国家自然科学基金(60174024);; 德国DFG资助项目
【CateGory Index】: Q813
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1 XIE Da-xing ,WU Jian-hong ,YU Yuan ,G ONG Jian-ping * Molecular Medical Center,Tongji Ho spital ,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,P.R.China;Quantitative Analysis of Cell Cycli n E Expression Threshold[J];Chinese Journal of Cancer;2002-07
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