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《ACTA AGRICUL TURAE SHANGHAI》 1995-01
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THE OPTIMIZATION OF EMBRYOID FOR ARTIFICIAL SEED OFApium graveolens

Ni Dexxiang; Deng Zhilong; Cen Yiqun ;Jiang Rumin; Yang Guangxiao; Yu Jing (School Of Life Science, Fudan University, Shanghai 200433,China. )  
Calli were induced from different.explants such as natural seed, leaf, cotyledon of Apium graveolens on MS media supplemented with lmg/L 2,4-D and 0. 5mg/L KT.The somatic embryos were produced by inoculating the callus from cotyledon into hormonefree MS medium, and were constructed into artificial seeds. This kind of artificial seeds obtained the highest conversion rate and the lowest deformation rate among those kinds of artificial seeds from different explants. The SDS-PAGE electrophoresis result indicated that the protein ingredient of callus from cotyledon was less than other kinds of calli. Maybe, the lack of 86kD, 63kD, 17kD, 15kD, 13kD resulted in the high quality of artificial seeds from cotyledon. In addition, when ABA was applied at the preferable concentration ling/L to treat the callus from cotyledon, the concentration of protein was increased from 1. 3mg/g (fresh weight) to 2. 9mg/g. The artificial seeds constructed from ABA-treated embryoids gained 56. 1 % conversion rate after 30 days low temperature storage. These results indicated the suitability and necessity of optimization of embryoids.
【Fund】: 上海市科委自然基金 高等学校博士点基金
【CateGory Index】: S336
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1 WU Weiguo;CAI Ziping;WANG Guoxiang;MI Yongwei;Institute of Chinese Herbal Medicines,Gansu Academy of Agricultural Sciences;Gansu Provincial Engineering Laboratory for Genetic Improvement and Quality Control of Chinese Herbal Medicine;Gansu Provincial Engineering Center for Domestication and Seeling Propogation of Rare Chinese Herbal Medicine;;Effects of Growth Regulator Root-soaking on Greenhouse-cultivated Angelica Seedlings Emergence and Growth Progress[J];中国现代中药;2017-11
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