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《ACTA AGRICUL TURAE SHANGHAI》 1995-01
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ESTABLISHMENT AND APPLICATION OF INSECTICIDAL ANTIBIOTICS BIOASSAY USING NEMATODES

Ma Chenzhu; Gu Zhenrong; Qian Zhenguan;Qian Guochen; Zhou Ainong; Zhang Fanqin (Plant Protection Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China. )  
A simple and rapid process using nematodes for bioassay of insecticidal substances from actinomycetal strains propagated through both slant culture and fermentation broth culture was developed. Languor and curl were the typical symptoms of nematodes (Panagrellus redivivus)exposed to the substances of Avermectins. Contrarily,the symptoms caused by piericidins were tumble and rigidity. Among the six nematode species tested,P. redivivus and APhelenchoides composticola showed more tolerance to Invermectin than the others. There were no evident effects on the vigor of nematodes (P. redivivus)when the concentrations of actetone,ethanol and methanol were below 3% in the assay system. According tothe nematocidal process from knockdown to death caused by insecticidal substances tested,the replacement of Lc50 with Kc50 could achieve bioassay results faster and more accurately.
【CateGory Index】: S482.7
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