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Liu Baoshen; Yin Liqing; Zhang Jianjun(Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.)  
When applied to 4 rice entries at various concentrations by addition to induction medium, differentiation medium or both of them, silicon was found baying effect of increasing callu induction frequency, plantlet differentiation frequency, green plantlet number per shoot cluster and transplanting survival rate in anther culture. The treatment of adding 60 mg. L- 1 silicon to both induction and differentiation media had the best effect among the six treatments, in this treatment the regeneration frequency of green plantlets for 02428,02428/IR36 and IR36/02428 significantly increased by 73. 9%, 86. 6 %and 79. 6 %,respectively, compared with the control. The treatment of adding 60 mg. L-1silicon to differentiation medium gave 13. 2% ~25. 5% more green plantlets than that to induction medium. Silicon in induction medium had different acting modes on different genotypes, so it might increase callus induction and plantlet differentiation frequencies of 02428/IR36 and IR36/02428 but was futile in increasing the callus induction frequency of 02428; however the calli induced from silicon-containing medium had a higher differentiation rate as compared to the control. Silicon is one of the major factors affecting rice anther culture, but isn' t a determinative factor.
【CateGory Index】: S511.104.3
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