APPLICATION OF ORTHOGONAL DESIGN IN EXPERIMENTS ON CALLUS CULTURE FOR Asparagus officinalis
Zhou Zhenglai; Lu Shihua(Plant Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106,China.)
The experiments on the ratio of auxins to cytokinins for the induction and growth of calli of Asparagus officinalis and on the transformation of embryonic calli were conducted by the orthogonal design in this study. The results indicated that the optimal factors and related levels were as follows: (a) the ratio of NAA to BA was 2: 1 ; (b)the initial amount of BA was 1mg .L-1 in MS medium;(c) AgNO3 and 3 amino acids(Asn+Gln and Pro)did not show any significant promoting effects on transformation of embryonic calli.
【CateGory Index】： S644.603.5