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《》 1993-01
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ESTABLISHMENT OF A XENOTRANSPLANTED HUMANCOLONIC TUMOR IN NUDE MICE AND ITS TARGETINGTREATMENT WITH IMMUNOTOXIN

Ge Hailiang Zhang Huizhen Zhou Guangyan Shanghai Second Medical Univsrsity, Shanghai Institute of ImmunologyXu Long Yangpu District Central Hospital  
An experimental tumor model was established in nude mice with subcutaneous innoculation of LoVo cells, a human colonic tumor cell line. It was observed that the tumor xenograft could be stably passed down through generations with a hundred percent viability. By using techniques of immunohistochemistry and ultrastructure analysis, it was further revealed that the characteristics of the xenotransplanted tumor were similar to the original ones in terms of morphological and pathological features, biological markers, as well as immunological functions. In addition, we were able to show that the tumor in the nude mice could be localized exactly with 131I-labelled anti-CEA MAb. Based on this observation an immunotoxin developed by conjugating the CEA MAb with Trichosanthin, a cell-toxic plant protein purified from a Chinese medicinal herb, was tested for targeting treatmtnt of the tumor xenograft, which resulted in 70% suppression of the tumor growth.
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【References】
Chinese Journal Full-text Database 1 Hits
1 Huang Ping 1 Lou Rongcan 1 Zhang Xuedong 1 Zhou Xigeng 2 ( 1 Department of Colorectal Cancer, Zhejiang Provincial Oncology Hospital, Hangzhou 310022; 2 Department of General Surgery, Ruijin Hospital, Shanghai Second Medical University);Nude Mice Model of Human Colon Cancer Liver Metastasis with Splenectomy[J];SHANGHAI LABORATORY ANIMAL SCIENCE;1999-03
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 LI Ning 1,DUAN Guang cai 2,LI Su yi 3,XI Yuan lin 2 (1.Henan Provincial Health & Anti epidemic Center,Zhengzhou 450003,China;?2.Medical College of Zhengzhou University, Zhengzhou 450052,China;?3.Department of Oncology,Zhongda Hospital,Southeast University,Nanjing 210009,China);Establishment of liver metastasis model from preventriculus squamous carcinoma in mice[J];Journal of Southeast University;2003-05
2 HUANG Ping *, ZHOU Xigeng, ZHU Chengmo, et al * Department of colorectal cancer, Zhejiang provincial oncology hospital,Hangzhou 310022, China;Experimental study in the prevention and therapy of liver metastases after curative resection of colorectal cancer with ~(131)I (anti CEA)MoAb[J];CHINESE JOURNAL OF NUCLEAR MDEICINE;2000-05
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