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APPLICATION OF DOT IMMUNOBINDING ASSAY IN AIIERGY DIAGNOSIS

Zhu Yinying Shen Lunan Hu Bingxiong Xu Yiping Renji Hospital,Shanghai Second Medical University (Shanghai 200025)  
A dot immunobinding assay (DIBA) used to measure three allergen-specific IgE antibodies simultaneously (D. farinae, House dust, Artemisia pollen) was established. To assess the usefulness of this method in the diagnosis of allergic disease,we compared the results of DIBA to those of ELISA in 50 atopic asthmatics. A good overall agreement was found (agreement = 90-92%) . There was also significant correlation betweea the titres of DIBA and those of ELISA (rs = 0.8987, p0.001). Eesults of the DIBA and the results of the skin test were also well correlated. (Agreement = 60.7-80.0%).This method is simple, easy to perform without expensive equipment, the results can be quantitated with a densitometer or just evaluated by visual comparison. The membrane absorbing allergens can be stored dry for several weeks without deterioration and used conveniently clinically.
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【References】
Chinese Journal Full-text Database 1 Hits
1 Chen Yonghong,Wu L anqiang,1Li Fangfang,Dong Ye,et al.( Guangxi lnstitute of National Medicine and Pharmacy,Nanning 530 0 0 1;1Nanning Municipal Second Peaple s Hospital);Clinical Study on Acupoint Pricking Blood Therapy in 31Cases of Allergic Rhinitis[J];Chinese Acuponcture & Moxibustion;1998-08
【Co-references】
Chinese Journal Full-text Database 1 Hits
1 Luo Dianxi, et al Department of Immunology;A Study on Total IgE in Serum and Nasal Secretion and Their Correction in Allergic Rhinitis[J];Journl of Guiyang Medical College;1993-02
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 Song Guihong 1,Zhang Qiang 2(1.Section of Acupuncture and Moxi bustion,Zaozhuang City Xuecheng District Hospital of TCM,Shandong 277000;2.Zaozh uang City Xuecheng District People′s Hos pital) [;Survey of Treatment of Allergic R hinitis with Acupuncture and Moxibustion in Recent Five Years[J];Chinese Acuponcture & Moxibustion;2001-06
2 CHEN Yong-hong, LU Lin , CHEN Hong , DONG Ye , WEI Jin~yu , LI Yan, Wei Tao (Zhuang Medicine Hospital of Guangxi, Nanning 530001, China; Guangxi Institute of Folk Medicine);Effects of pricking blood therapy on ACTH contents in the hypothalamus-pituitary-adrenal axis in the rat of experimental allergic rhinitis[J];Chinese Acupuncture & Moxibustion;2005-03
3 CHEN Jie,LAI Xin-sheng School of Acu-Moxibustion & Massage,Guangzhou University of TCM,Guangzhou 510405,China;The review of acu-moxibustion for allergic rhinitis in recent years[J];Journal of Clinical Acupuncture and Moxibustion;2005-08
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