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《Shanghai J.of Immunology》 1997-01
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Rapid Elution of Tumor Peptides from Human Lung Cancer Cells by Mild Acid Method

Deng Weiwen, Xie Hong (Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031)Claudia Semino, Gianni Melioli (Laboratory of Clinical Pathology, National Institute for Cancer Re-ssearch of Italy)  
Mild acid elution is a novel method to acheive a rapid elution of immuno-genic peptides from MHC class I-peptide complex on the surface of cultured cells. We use this method to elute MHC class I-presented, CD8+T cells-recognized tumor peptides from the surface of human non-small cell lung cancer cell line A549. Through systematically comparing the peptide elution rate and the viable cell rate in different elution conditions, we concluded that by using pH3.3 as the citrate-phos-phate buffer's pH, I min for the elution period, the best elution effect can be achiev-ed. We also found that MHC class I presented peptides re-expressed on the cell surfa-ce within 24 hrs after elution, so the cells may be daily eluted for 3 consecutive days with the cells' condition without being altered. Thus the output of the peptides was raised.
【Fund】: 世界实验室生物技术中心资助项目
【CateGory Index】: R734.2
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