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《Shanghai J.of Immunology》 1997-04
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Preparation and Application of the IL-13 cDNA Probe

Wang Wen, Sun Rui, Tian Zhigang, et al. (Shandong Cancer Biotherapy Center, Jinan250062)  
The gene expression of IL-13 on tumor cell lines were investigated with hIL-13 cDNA probe, which was labelled with biotin by nick translation. The results obtained by RNA dot blot and Northern blot showed that there were different degrees of IL-13 gene expressions on the tumor cells. The sensitivity of this probe was rather high with a visible colorations under 5~ 10pg of biotin-labelled probe dotted in NC membrane, and the specificity of the probe was also good. Ribonucleic acid (RNA) containing IL-13 mRNA hybridized with IL-13 cDNA probe and gave positive results, while RNA without IL-13 gene showed a negative result. The preparation of IL-13 probe lays foundation for further researches on the biological properties of IL-13.
【CateGory Index】: R392
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Chinese Journal Full-text Database 2 Hits
1 Wang Wen; Sun Na; Tian Zhigang; Wang Junpu; Liu Jie; Zhang Jianhua; Liu Zhongbin (Shandong Cancer Biotherapy Cealer Jinan 250062);Construction and Comparison of IL-13 Fusion-protein and Non-fusion-pro-tein Expression Vector[J];CHINESE JOURNAL OF BIOCHEMICAL PHARMACEUTICS;1997-05
2 Wang Wen,Tian Zhigang,Sun Rui et al.Shangdong Cancer Biotherapy Center,Jinan 250062;THE CONSTRUCTION OF hIL-13 FUSION EXPRESSION VECTOR AND THE EXPRESSION OF IL-13 PROTEIN IN E.coli[J];CHINESE JOURNAL OF IMMUNOLOGY;1998-05
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