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《Shanghai J.of Immunology》 2001-01
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Purification of Proto-oncogene Pim-1 Kinase-GST Fusion Protein and Analysis of Its Kinase Activity

LIU Cun lan 1,Masahiko HIBI 2,Toshio HIRANO 2(1 Department of Immunology,Fudan University Medical School,Shanghai 200032;2 Division of Molecular Oncology,Biomedical Research Center,Osaka University Medical School,Osaka 565,Japan)  
The human Pim 1 proto oncoprotein was expressed in Escherichia coli as a gluthione S transferase(GST) fusion protein and the enzymatic properties of its kinase activity were characterized in this study The Pim 1 GST fusion protein showed an optimal pH of 7 5 and optimal ion concentration of 10 mmol/L MgCl 2 Higher cation concentration and higher or lower pH were all inhibitory for kinase activity of Pim 1 GST fusion protein
【Fund】: 日本文部省研究基金
【CateGory Index】: R73-3
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