Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Shanghai J.of Immunology》 2001-01
Add to Favorite Get Latest Update

Induction of Proliferative Responses of Neonatal Cord Blood Lymphocytes by Costimulation of PMA and Ionomycin and Change of Intracellular Ca~(2+) Concentration

HE Zheng juan,CHEN Tong xin,WU Sheng mei,YING Da ming,ZHOU Xin, ZHU Ya zhong(Shanghai Institute for Pediatric Research,Xinhua Hospital,Shanghai Second Medical University,Shanghai 200092)  
To compare the proliferative responses between the neonatal cord blood lymphocytes and the peripheral blood lymphocytes and to study the changes of intracellular Ca 2+ concentration,stimulated by anti CD3 McAb,PMA and IM,the proliferative responses of lymphocytes were detected by 3 TdR incorporation and the Ca 2+ concentration of lymphocytes were examined by fluorospectrometry The experimental results showed that the prolipherative responses of both cord blood lymphocytes and the adult peripheral blood lymphocytes to anti CD3 antibodies or PMA+IM were significantly increased,but the responses of cord blood lymphocytes to anti CD3 antibodies was lower than those of the adult peripheral blood lymphocytes,and the responses of cord blood lymphocytes to PMA+IM were relatively close to those of the adult lymphocytes The intracellular Ca 2+ concentration also elevated in both cord blood and adult blood lymphocytes Because PMA+IM costimulation can partially improve the proliferative deficiency of neonatal cord blood lymphocytes,it suggests that defects on the signal transduction might be one cause of the neonatal immunodeficiency
【CateGory Index】: R392.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved