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《Shanghai J.of Immunology》 2001-03
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Comparison of Interleukin-4 Gene Expression and NF-AT Activity in Neonates and Adults Peripheral Blood Mononuclear Cells

JIANG Li ping,YANG Xi qiang,WANG Li jia,LI Cheng rong,WU Shi xiao(Children's Hospital,Chongqing University of Medical Sciences,Chongqing 400014)  
The mechanisms of lowered IL 4 gene expression in neonates was studied by comparing the differences of IL 4 gene expression and nuclear factor of activated T cell (NF AT) activities between neonatal cord blood mononuclear cells (CBMC) and adult PBMC The levels of IL 4 and its mRNA were detected by using ELISA and RT PCR respectively,and the activity of NF AT was detected by using electrophoresis mobility shift assay (EMSA) Results showed that,after stimulation with anti CD3 and PMA,the concentrations of IL 4 in supernatants of CBMC were significant lower than those in PBMC In our test system,the activities of NF AT in CBMC were almost undetectable,but stronger in PBMC The results of this study suggest that lack of the regulation by NF AT at transcription level may be associated with insufficient transcription of IL 4 gene in neonates,and thus result in insufficient IL 4 gene expression in neonates
【Fund】: 卫生部科研课题基金资助项目 (No 94 1 3 5 4)
【CateGory Index】: R392.11
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【References】
Chinese Journal Full-text Database 2 Hits
1 JIANG Hong, ZHANG Qiuye, WANG Shujie, et al\ Department of Pediatrics, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003\;;A STUDY OF FUNCTIONAL BAIANCE OF Th1-Th2 IN CORD BLOOD MONONUCLEAR CELLS OF FULL-TERM NEONATES[J];Acta Acadimae Medicinae Qingdao;2002-03
2 Wu Shenrong,Xie Zhaohui,He Shuangteng,et al (Hunan College of Traditional Chinese Medicine, Changsha 410007);Effects of Qiweibaizhu Powder on NK, IFN-γ and IL-4 of Suckling Mice Infected with Human Rotavirus[J];Chinese Journal of Information on TCM;2002-04
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Chen shi et al. (Children's Hospital,Chongqing University of Medical Science);EFFECTS OF CYTOKINES ON FUNCTION OF NEONATAL B LYMPHOCYTES[J];THE JOURNAL OF NEONATOLOGY;1997-06
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Jian,HAN Ming xiang,ZHANG Wan dong,CHEN Guang liang,LEI Li (The First Hospital Affiliated to Anhui College of Traditional Chinese Medicine,Anhui Hefei 230031,China);INFLUENCE OF XINFENG CAPSULE ON SERUM LEVEL OF IL-1, TNFα,IL-4,AND IL-10 IN ADJUVANT ARTHRITIS RATS[J];Journal of Anhui Traditional Chinese Medical College;2002-02
2 HE Yan-hua1,2,FANG Yong-xiang2,CHEN Guo-hua2,DUAN Feng-yun2,WANG Sheng-fu1,2,LI Xiao-qing2,JING Zhi-zhong1,2(1.Gansu Agricultural University,Lanzhou,Gansu,730070,China;2.Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences;Key Laboratory of Veterinary Parasitology of Gansu Province,State Key Laboratory of Veterinary Etiological Biology,Lanzhou,Gansu,730046,China);Cloning and Sequence Analysis of Yak Interleukin-4 gene[J];Progress in Veterinary Medicine;2008-08
3 CHEN Wei,CHEN Wei-xiong,JIN Yong,LU Yun-min (Department of Gastroenterology,Shanghai No.6 People’s Hospital,Shanghai Jiaotong University,Shanghai 200233,China);Effect of ligustrazine on the expression of TNF-α、IL-4 and IFN-γin rats with liver fibrosis[J];World Clinical Drugs;2010-03
4 Hou Wei, Lei Chun lian. The Second Hospital, Xi an MedicalUniversity, Xi-un 710004;Preliminary approach on the determination of serum II. I content and its clinical significance in children with pneumo-nia[J];The Journal of clinical Pediatrics;1998-01
5 XU Jincheng1 WANG Shufei2 XIA Xiaoming1 WANG Shengchun3 CHE N Qun3 (1 Department of Cardiology, Affiliated Hospital of Guang dong Medical College, Zhanjiang 524001; 2Department of Geriatrics, A ffiliated Hospital of Guangdong Medical College; 3De;Detection of serum levels of interleukin 4 and 10 in patients with hypertension as well as its clinical significance[J];Journal of Clinical Cardiology;2001-08
6 Zhang Qing et al(Department of Rheumatism Immunity the First Hosppital Affiliated to Jiamusi University Jiamusi Heilongjiang 154003);EFFECTS OF LIGUSTRAZINE PHOSPHATE ON SERUM CYTOKINES TNF ALPHA, IL-4 AND IL-10 LEVELS IN RATS WITH ADJUVANT ARTHRITIS[J];Journal of Mudanjiang Medical University;2010-06
7 HE Qingwei,WANG Jiacai,PENG Guoguang,WANG Faxiang Department of Neurology,First affiliated hospital,Chongqing Medical University,Chongqing 400016,China;Construction and expression of fusional eukaryotic expression vector PVAX1-IL-4/SYN-B[J];Immunological Journal;2009-05
8 SUN Su-ping,ZHOU Cui-ying,ZHOU Hai-rong,Chen Yu-zhen (Affiliated Hospital of Shandong University of TCM, Jinan 250011, China);INFLUENCE OF BISUQING MIXTURE UPON SERUM CYTOKINE LEVELS IN RATS WITH ADJUVANT ARTHRITIS[J];Journal of Shandong University of Traditional Chinese Medicine;2003-06
9 JIANG Li ping,YANG Xi qiang,WANG Li jia, LI Cheng rong, WU Shi xiaoImmunologic Laboratory, Children's Hospital, Chongqing University of Medical Sciences, Chongqing 400014, China;Interleukin-4 gene expression and its regulation in cord blood T cells[J];JOURNAL OF CELLULAR AND MOLECULAR IMMUNOLOGY;2000-04
10 Xu Su-qin,Bao Zhao-sheng,Wu Zhong-min,Wang Yong,Qi Yong-xiao,Zhang Jian-xing School of Medicine,Taizhou College,Taizhou 318000,Zhejiang Province,China;Changes in pathomorphology of synovial membrane tissues and levels of serum interleukin 4,interleukin 10 in adjuvant-induced arthritis rats following acupuncture treatment[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2008-28
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Qiuye , SHAN Yanchun , DONG Zengyi, et al\ Department of Pediatrics, The Affiliated Hospital of Qingdao University Medical College , Qingdao 266003\;;A PRELIMINARY OBSERVATION OF T-LYMPHOCYTE APOPTOSIS AND ITS EFFECTING FACTORS IN FULL-TERM NEONATES[J];Acta Acadimae Medicinae Qingdao;2001-04
2 WU Kui, BI Yu-tian, SUN Kun, XIA Jun-bo, WANG Chang-zheng (Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China);Comparative study of allergic pulmonary inflammation of BALB/c and C57BL/6 mice sensitized and challenged with house dust mite extracts[J];Acta Academiae Medicinae Militaris Tertiae;2005-17
3 Xiong Ran, Wang Yanfang, Fei Ying, et al. Affiliated Hospital of Guiyang Medical College, Guiyang 550004;The changes and relationship of newborn cord blood IgE, IL-4, IL-10 in infants with allergic diseases[J];Guizhou Medical Journal;2003-04
4 Guo Guimei,Guo Luzhou,Wang Xiaochuan,He Wanlin.Department of Pediatrics,The Children's Hospital of Fudan University,Shanghai 200032;Relationship between umbilical serum IgE level as well as PPD skin test and anaphylactic diseases in children[J];The Journal of clinical Pediatrics;2002-03
5 HAO Yu,QIU Quanying,WU Jun (Department of Immunology,Beijing University of Chinese Medicine,Beijing 100029,China);Effect of astragalus polysaccharin(APS)on lymphocyte-endothelium adhesion and the molecular mechanism[J];IMMUNOLOGICAL JOURNAL;2000-03
6 Wan Xing Liu Qianxian Fang Xunxin, et al.College of Basic Medical Science,Guangzhou University of TCM 510407;Regulatory Effects of Huangqi Jianzhong Tang on lmmunologic Function of Mice with Spleen-deficiency Syndrome[J];JOURNAL OF GUANGZHOU UNIVERSITY OF TRADITIONAL CHINESE MEDICINE;1997-04
7 JIANG Li-xue 1,SHI Gui-ying 2,SHI Xue-geng 2,XU Yi-ping(1. Department of Respiratory Diseases,Renji Hospital,Shanghai Second Medical University, Shanghai 200001;2. Department of Biophysics,Shanghai Second Medical University,Shanghai 200025);Study on Change of the CD4~+ and CD8~+T Cell Populations in Peripheral Circulation Producing IFN-γ and IL-4 in Neonates,Children and Adults[J];Shanghai J.of Immunology;2002-01
8 Zhang Aihua,Chen Ronghua,Wu Yuanjun, Pan Xiaoqin ,Huan Wnyan, Cai Yi Center of Pediatric Nephrology, Nanjing Medical University,Nanjing 210029;THE EXPRESSION OF IL 13 AND ITS RELATIONSHIP WITH PATHOLOGICAL TYPES IN CHILDREN WITH NEPHROTIC SYNDROME[J];CHINESE JOURNAL OF NEPHROLOGY, DIALYSIS & TRANSPLANTATION;1999-05
9 CHEN Chun, HUANG Shao-liang, WU Yan-feng (The Hematopoietic Stem Cell Transplantation Center of Department of Pediatrics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou 510120,China);Levels of cytokines in human umbilical cord blood serum and in cultured mononuclear cells isolated from cord blood induced by phytohemagglutinin and lipopolysaccharide[J];Chinese Journal of Pathophysiology;2002-04
10 ZHONG Zhao jin, LIU Jun (Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050, China);Study Progresses in Triterpenes of Poria cocos and Its Pharmacology[J];Chinese Traditional Patent Medicine;2001-01
【Secondary References】
Chinese Journal Full-text Database 4 Hits
1 SHEN HUI,XU LUO(Department of Pathophysiology,Qingdao University Medical College,Qingdao 266021,China);THE EFFECT OF 17β-ESTRADIOL ON CEREBROCELLULAR APOPTOSIS IN THE CA1 PYRAMIDAL CELL LAYER OF HIBD RATS[J];Acta Academiae Medicinae Qingdao Universitatis;2008-05
2 GUO Wei-jun(TCM Hospital of Liuyang,Liuyang,Hunan 410300,China);Effect of modified Qiwei Baizhu powder on irritable bowel syndrome with weakened spleen and stomach[J];Journal of Traditional Chinese Medicine University of Hunan;2010-09
3 JIANG Xiao,WU Can-rong,TIAN Xue-fei,et al(Hunan University of TCM,Changsha 410007,China);Protective Effect of Qiweibaizhu Powder against HRV Infection of Intestinal Mucosal Epithelial Cells of Suckling Mice[J];Chinese Journal of Information on Traditional Chinese Medicine;2010-05
4 LU Yu,ZHANG Jin-qiu,DENG Bi-hua,YU Zhe,LV Fang,HOU Ji-bo(National Research Center of Veterinary Biologicals Engineering and Technology,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China);ENHANCEMENT OF CPG ODN ON IMMUNE RESPONSES OF INACTIVATED NEWCASTLE DISEASE VIRUS VACCINE[J];Chinese Journal of Animal Infectious Diseases;2010-06
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