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《Shanghai J.of Immunology》 2003-01
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Expression of hCGβ-C3d Fusion Protein in CHO Cells

WANG Ming yan 1,LI Lei 1,LI Da jin 1,SHEN Qing xiang 2,ZHU Ying 1(1 Laboratory for Reproductive Immunology,Institute of Obstetrics & Gynecology,Fudan University,Shanghai 200011;2 Shanghai Institute of Parenthood Planning Research,Shanghai 200032,China)  
To construct a new plasmid to enhance the immunogenicity of hCGβ with potent molecular adjuvant C3d,a plasmid pcDNA3 hCGβ C3d3 was constructed with the fusion protein determined and analyzed from transfected CHO cells by methods of immunohistochemistry and Western blotting It was found that the stable expression system of CHO cells transfected by this plasmid could secret the recombinant fusion protein hCGβ C3d3 at a speed of 660 ng/10 6 cells/48 hrs The expression products contained both C3d receptor binding activity and hCGβ antigenicity as detected by Raji cell immunohistochemical assay There appeared as 3 protein bands in the supernatants of cultures of transfected cells bearing anti hCGβ activity on SDS PAGE and Western blots with molecular weight of 120,90 and 65 kD respectively It concludes that we have successfully constructed a plasmid pcDNA3 hCGβ C3d3 that is able to express the fusion proteins in eukaryotic cells and may provide a useful tool for further researches in hCGβ contraceptive DNA and protein vaccines
【Fund】: 国家自然科学基金资助项目 (No .3 0 2 712 3 5 );; 计划生育药具国家重点实验室资助项目 (No B2 0 1 1);; 上海市自然科学基金资助项目 (No 0 0ZB14 0 5 8);; 教育部博士学科点专项科研基金资助项目 (No 9941);; 卫生部优秀青年人才专项科研基金资助项目(No 97
【CateGory Index】: R392.3
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Chinese Journal Full-text Database 5 Hits
1 ZHANG Li, WU Yu-zhang (Institute of Immunology, College of Medicine, Third Military Medical University, Chongqing 400038, China);Construction and eukaryotic expression of recombinant plasmid βhCG-CTP/pcDNA3[J];Acta Academiae Medicinae Militaris Tertiae;2005-01
2 YU Min,LI Da-jin,WANG Xiu-li,YUAN Min-min,ZHU Ying,YAO Xiao-ying,LI Hua-ping(Laboratory of Reproductive Immunology,Institute of Obstetrics and Gynecology,Fudan University,Shanghai 200011,China);Immunization hCGβ-C3d3 DNA promotes selectively the clonal expansion of B cell proliferation in BALB/c mice[J];Current Immunology;2006-01
3 WANG Xiu-li,LI Da-jin,YUAN Min-min,ZHU Ying Institute of Obstetrics & Gynecology,Fudan University,Shanghai 200011;The comparison of the expression efficiency of hCGβ-C3d3 fusion protein in the eukaryotic vector phCMV1 and pcDNA3 transfected CHO cells[J];Journal of Reproductive Medicine;2004-04
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【Secondary References】
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