Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Shanghai J.of Immunology》 2003-01
Add to Favorite Get Latest Update

Expression of hCGβ-C3d Fusion Protein in CHO Cells

WANG Ming yan 1,LI Lei 1,LI Da jin 1,SHEN Qing xiang 2,ZHU Ying 1(1 Laboratory for Reproductive Immunology,Institute of Obstetrics & Gynecology,Fudan University,Shanghai 200011;2 Shanghai Institute of Parenthood Planning Research,Shanghai 200032,China)  
To construct a new plasmid to enhance the immunogenicity of hCGβ with potent molecular adjuvant C3d,a plasmid pcDNA3 hCGβ C3d3 was constructed with the fusion protein determined and analyzed from transfected CHO cells by methods of immunohistochemistry and Western blotting It was found that the stable expression system of CHO cells transfected by this plasmid could secret the recombinant fusion protein hCGβ C3d3 at a speed of 660 ng/10 6 cells/48 hrs The expression products contained both C3d receptor binding activity and hCGβ antigenicity as detected by Raji cell immunohistochemical assay There appeared as 3 protein bands in the supernatants of cultures of transfected cells bearing anti hCGβ activity on SDS PAGE and Western blots with molecular weight of 120,90 and 65 kD respectively It concludes that we have successfully constructed a plasmid pcDNA3 hCGβ C3d3 that is able to express the fusion proteins in eukaryotic cells and may provide a useful tool for further researches in hCGβ contraceptive DNA and protein vaccines
【Fund】: 国家自然科学基金资助项目 (No .3 0 2 712 3 5 );; 计划生育药具国家重点实验室资助项目 (No B2 0 1 1);; 上海市自然科学基金资助项目 (No 0 0ZB14 0 5 8);; 教育部博士学科点专项科研基金资助项目 (No 9941);; 卫生部优秀青年人才专项科研基金资助项目(No 97
【CateGory Index】: R392.3
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 5 Hits
1 ZHANG Li, WU Yu-zhang (Institute of Immunology, College of Medicine, Third Military Medical University, Chongqing 400038, China);Construction and eukaryotic expression of recombinant plasmid βhCG-CTP/pcDNA3[J];Acta Academiae Medicinae Militaris Tertiae;2005-01
2 YU Min,LI Da-jin,WANG Xiu-li,YUAN Min-min,ZHU Ying,YAO Xiao-ying,LI Hua-ping(Laboratory of Reproductive Immunology,Institute of Obstetrics and Gynecology,Fudan University,Shanghai 200011,China);Immunization hCGβ-C3d3 DNA promotes selectively the clonal expansion of B cell proliferation in BALB/c mice[J];Current Immunology;2006-01
3 WANG Xiu-li,LI Da-jin,YUAN Min-min,ZHU Ying Institute of Obstetrics & Gynecology,Fudan University,Shanghai 200011;The comparison of the expression efficiency of hCGβ-C3d3 fusion protein in the eukaryotic vector phCMV1 and pcDNA3 transfected CHO cells[J];Journal of Reproductive Medicine;2004-04
4 WANG Xiu-Li,LI Da-Jin,YUAN Min-Min,WANG Ming-Yan,ZHU Ying,MENG Yi.Laboratory of Reproductive Immunology, Institute of Obstetrics and Gynecology, Fudan Unive rsity, Shanghai 200011,China [;The expression, characterization and purification of the secreted hCGβand hCG β-C3d3 fusion protein in CHO cells[J];Chinese Journal of Immunology;2004-11
5 WANG Xiu-Li,LI Da-Jin,YUAN Min-Min,WANG Ming-Yan,ZHU Ying,MENG Yi.Laboratory of Reproductive Immunology,Institute of Obstetrics and Gynecology,Fudan University,Shanghai 200011,China;Enhancement of antibodies to the hCGβ contraceptive vaccine by using the molecular adjuvant C3d3[J];Chinese Journal of Immunology;2005-09
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Tao Sanju D.B.Boyle G.L.Ada Institute of Virology, Chinese Academy of Preventive Medicine.Department of Microbiology, John Cutrin School of Medical Research Canberra, Australia.;IMMUNE RESPONSE IN MICE TO RECOMBINANT VACCINIA VIRUS EXPRESSING HEMAGGLUTININ OF INFLUENZA VIRUS[J];Chinese Journal of Virology;1986-04
2 CHEN Yun LIU Zhe PENG Jing-Pian ZHANG Fu-Chun CHEN You-Zhen WANG Bin (State Key Laboratory of Reproductive Biology, Institute of Zoology, The Chinese Academy of Sciences, Beijing 100080, China);EXPRESSION OF BETA SUBUNIT OF RHESUS MONKEY CHORIONIC GONADOTROPIN (rmCGβ) DNA IN HeLa CELLS AND THE IMMUNE RESPONSES IN BALB/C MOUSE INOCULATED BY rmCGβ DNA VACCINE[J];Acta Zoologica Sinica;2001-04
3 Zhang Shaolun, Cui Xinjiang (Department of Microbiology);IMMUNOLOGICAL STUDY ON THE SUPPRESSIVE EFFECT OF TRIPTERYGIUM REGELLI ON COLLAGEN-INDUCED EXPERIMENTAL ARTHRITIS II. NFLUENCE OFT. REGELLI ON THE LEVEL OF ANTI-COLLAGEN IMMUNE RESPONSE IN MODEL ANIMALS[J];;1990-02
4 Bai Xue,Yang Jie,Liu Changfu,et al.School of Pharmacy,Guiyang Medical University,Guiyang 550004,China;The anti-aging effect of Corydalis yanhusuo W.T.Wang on aging mice induced by D-galactose[J];Guizhou Medical Journal;2008-05
5 GAO Yu-gang, ZHENG Quan, DU Rui, WANG Quan-kai, WANG Shu-zhi ( Chinese Medicinal Material College, Jilin Agricultural University, Changchun 130118, China);Isolation and RT - PCR Identification of Bovine Viral Diarrhea Virus from China Sike Deer[J];Journal of Economic Animal;2004-02
6 Pan Shanpei Zhu Weiiie Tan Chi Liu Xuegao (Center for Reproductive Immunology Research);TOXICOLOGY AND SAFETY STUDIES OF HUMAN CHORIONIC GONADOTROPIN (hCG) CONTRACEPTIVE VACCINE ON RHESUS MONKEY[J];Journal of Jinan University;1992-01
7 LI Wei-tao,\ PAN Shan-pei,\ XIE Qi-xuan,\ XIAO Luan-juan,\ LU Chun-bin (The Center For Reproductive Immunology Research, Jinan University, Guangzhou 510632, China);Construction of recombinant plasmid pPIC9K-pZP3α hCGβ CTP~(109~145)[J];Journal of Jinan University(Natural Science & Medicine Edition);2002-03
8 TIAN Ju-xia~(1),SHI Li-yun~1,ZHOU Xie-lai~1,LOU Zhong-ming~2(1.Medical College of Hangzhou Teachers College,Hangzhou 311207,China;2.Hangzhou Xiaoshan the First People's Hospital,Hangzhou 310018,China);THE EFFECT OF MURINE HEPATOMA CELLS WITH DIFFERENT METASTATIC ABILITY ON IMMUNOLOGICAL ROLES OF PERITONEAL MACROPHAGES[J];Acta Anatomica Sinica;2007-01
9 LU Xue chun, LOU Fang ding, HAN Wei dong, XU Zhou min, MU Yiming YU LI (Hematology Department, PLA General Hospital, Beijing 100853, China);Sub-cloning of LRP16 gene promoter and constructing of expre ssion regulatory vectors[J];Academic Journal of Pla Postgraduate Medical School;2003-02
10 G.P. Talwar (International Centre for Genetic Engineering and Biotechnology, Aruna asaf Ali Marg, New Delhi, India; Talwar Research Foundation, E 6, Neb Valley, Neb Sarai, New Delhi, India) [WT5”HZ];Vaccines and Passive Immunological Approaches for the Control of Fertility and Hormone dependent Cancers[J];ACTO ANDROLOGICA SINICA;2000-02
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 LU Dong-ming,SHAO Jia-hui (Immunology Department,Medical College of Qinghai University,Xining 810001,China);The Molecular Adjuvant PHA Increases the Effect of Specific Humoral Immunity[J];Journal of Qinghai Normal University(Natural Science Edition);2006-02
2 Lu Dongming,Shao JiahuiImmunology Department,Qinghai University Medical College;THE MOLECULAR ADJUVANT HIL-2 INCREASES THE IMMUNE EFFECT OF PFC AND QHS IN MICE[J];Journal of Qinghai Medical College;2006-01
3 Yan SHI~(1,2) Shu JIANG~2 Wei-ying SHEN~2 Ya-hong JIANG~2 Qing-xiang SHEN~2 (1.Shanghai Medical College,Fudan University,Shanghai,200032) (2.Shanghai Institute of Planned Parenthood Research,Shanghai,200032);Expression and Purification of the Fusion Proteins hCGβ-(CTP)_4 and hCGβ-(CTP)_4-(C3d-P28)_4 in E.coli[J];Reproduction & Contraception;2007-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved