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《Shanghai J.of Immunology》 2003-01
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The Correlation between the HBcAg Content and Its Cellular Apoptotic Rate as Well as the Expression of Apo2.7 Factor in Liver Needling-Biopsy Tissue with CHB by Flow Cytometric Analysis

YAN Yu ping 1,KAN Xiao 1,CHANG Shu zhen 1,ZHU Tong yu 2,GUO Chao ying 1(1 Jinan Infective Disease Hospital,Jinan 250021;2 Jinan Military General Hospital,Jinan 250031,China)  
To investigate the correlation between the HBcAg content and its cellular apoptotic rate (CAR),as well as the expression of Apo2 7 factor in liver needling biopsy tissue with chronic hepatitis B (CHB),thirty seven patients with CHB were performed liver needling biopsy and the tissues were made into cellular suspension Then immunologically marked by fluorescent monoantibody of HBcAg and Apo2 7 (a mitochondral membrane protein),as well as painted by AnnexinV FITC/Propidium iodide(PI) and assayed its CAR,which could distinguished early apoptotic cells from necrotic cells All were quantitatively measured by flow cytometry(FCM) At the same time the common HE sections were pathologically examined The results were that:there was different percentage of apoptotic cells from necrotic cells in liver needling biopsy tissue with CHB The percent of necrotic cells and CAR in III degree damage patients were statistically more than that in I degree damage ones ( P 0 01) The HBcAg content was statistically correlated with its CAR and the expressive level of Apo2 7 factor This article suggested that the analysis of CAR and the expressive level of Apo2 7 factor could directly determine the tissue damage degree and detected curate effect,which might has important clinic significance
【CateGory Index】: R512.62
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