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《》 2003-04
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Effects of Dendritic Cells in the Active Immunoprophylactic Action of Elemene Combo Tumor Cell Vaccine

QU Hong,GUO Lian ying,SHI Guang xia,LI Dan,PIAO Hua,SHEN Jie,QIAN Zhen chao(Institute of Biotherapy for Tumors,Dalian Medical University,Dalian 116027,China)  
Mice were immunized with HCa F hepatocarcinoma elemene combo tumor cell vaccine(H TCV),L615 leukemia elemene combo tumor cell vaccine(L TCV),the lysates of the corresponding tumor cells and Corynebacterium parvum (CP).The dendritic cell(DC) rich speen cells of mice were isolated and were pulsed with tumor cell lysates of HCa F(H)or L615(L),TCV cell lysate of H TCV (TH) or L TCV Their activity to induce proliferation of normal non adherent spleen cells was measured with MTT assay It found that the proliferation of spleen cells stimulated with DC pulsed with TCV cell lysates from mice pulsed with TCV or TCV cell lysates was much more potent than those of DC pulsed with tumor cell lysates from mice of not immunized Injection of CP to mice DC could further increase this effect
【Fund】: 国家自然科学基金重点资助项目 (No 3 973 0 44 0 II)
【CateGory Index】: R730.3
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