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《》 2003-04
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Study on the Expression and Immunogenicity of SmB/B' Epitope

WU Jin,WU Hou sheng,XIONG Si dong(Department of Immunology,Key Laboratory of Molecular Medicine Ministry of Education,Shanghai Medical College of Fudan University,Center for Gene Immunization and Vaccine Research,Shanghai 200032,China)  
To construct the eukaryotic expression vector of SmB/B' epitope and to study its immunogenicity after its expression in vivo and in vitro,the target DNA encoding the SmB/B' epitope was cloned by RT PCR,and then the eukaryotic expression vector pcDNA3 f SmB/B' was constructed Its expression and antibody binding activities were detected in vitro,and its immunogenicity was determined by gene immunization in vivo It was found that the eukaryotic expression vector pcDNA3 fSmB/B' was constructed correctly,and it could be expressed in vitro with binding activity with the anti SmB/B' antibody All BALB/c mice immunized with this plasmid DNA produced anti SmB/B' antibody The experimental results indicate that the constructed eukaryotic expression vector of SmB/B' epitope can efficiently express in vitro and in vivo and the expressed epitope has immunogenicity
【Fund】: 国家自然科学基金资助项目 (No 3 9970 70 0 )
【CateGory Index】: R392.3
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