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《》 2003-04
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Expression of HLA Class I Molecules in Oral Squamous Cell Carcinoma Cell Lines

TANG Qiu sha 1,ZHANG Jian qiong 1,QI Bin 2,SHEN Chuan lai 1,LU Xiao xuan 1,BAO Hai xun 1,Soldano F 3,WANG Xin hui 3,XIE Wei 1(1 Research Center of Genetics,Southeast University,Key Laboratory of Gene Diagnosis and Therapy in Jiangsu Province,International“HLA Expression and Cancer" Reference Laboratory,Nanjing 210009,China;2 Department of Pathology,Jiangsu Oral Hospital,Nanjing 210006,China;3 Department of Immunology,Roswell Park Cancer Institute,Buffalo,NY 14263,USA)  
To investigate the mechanism of down regulation of HLA class I molecules on two cell lines of oral squamous cell carcinoma,the expressions of HLA class I molecules were determined by flow cytometry and Western blotting assay using a panel of antibodies To characterize the down regulation of HLA molecule,the analysis was extended to RNA level by RT PCR by using the specific primers It was found that the expression of HLA class I molecules of these two tumor cell lines was reduced in comparison with those of the normal epithelial cell line (Hacat) as determined with flow cytometry and Western blotting The results by using RT PCR showed that the expressions of HLA A,B,C,LMP2,LMP7 and LMP10 gene mRNA were down regulated in case of the Tca8113 cell line,but in case of KB cell line,the expressions of HLA A,B,C,LMP7,LMP10 gene mRNA were down regulated with marked reduction or deletion of the LMP2 and PA28α gene mRNA,yet,those of the TAP1,TAP2,Tapasin gene mRNA showed no any changes Treatment with IFN γ could enhance the expressions of HLA A,B,C,LMP7,LMP10 transcripts and restore those of LMP2 and PA28 transcripts It concludes that the abnormal expressions of HLA class I molecules in oral squamaous cell carcinoma cell lines may be presumably due to the low efficiency of HLA gene transcription
【Fund】: 教育部高等学校优秀青年教师教学科研奖励计划资助项目 (教人司 2 0 0 1/ 182 )
【CateGory Index】: R739.8
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