Effects of IL-4 on the Production of IL-12 by Dendritic Cells

DING Chuan lin 1, YAO Kun 1, ZHANG Tian tai 2, ZHOU Feng 1(1 Department of Microbiology and Immunology, Nanjing Medical University, Nanj ing 210029,China;2 First Affiliated Hosipital of Lanzhou Medical College,Lanzhou 730000,China)  

To evaluate the effect of IL 4 on the ability of dendritic cells(DC) to produce IL 12 p70 and its regulatory mechanism, the mature mouse bone marrow derived DC were generated by cultivation of C57BL/6 mice bone marrow cells with GM CSF and IL 4 for 6 days and followed by addition of LPS ,TNF α or CpG ODN for 2 days The surface molecules of DC including CD80 and MHC class II molecules were analyzed by FACS, the stimulatory effect of DC on the proliferation of allogenic T lymphocytes was examined by 3 H TdR incorporation assay, and IL 12 p70 was quantified by enzyme immunoassay IL 12 p35 and p40 mRNA expressions and its changes were measured by RT PCR and real time PCR It was found that the mature DC could be obtained by these methods of study These mature DC showed a high level of expressions in CD80 and MHC class II molecules, and have capacity to stimulate proliferation of the naive T cells in vitro IL 4 strongly enhanced the production of bioactive IL 12 p70 in the presence of maturation stimulus Results by using RT PCR and real time PCR indicated that the effects of IL 4 on the production of IL 12 p70 might be mediated by the regulation of IL 12 p35 mRNA

【Fund】： 江苏省重点实验室开放基金资助项目 (No k2 0 3 0 )
【CateGory Index】： R392.12

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