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《》 2003-04
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Screening of CpG Oligonucleotide with Immunostimulatory Activities on Porcine Peripheral Blood Mononuclear Cells

MA Yan qin 1, LI Guang fu 1, ZHU Hong fei 2, SHAO Guo qing 3,ZHANG Zhao song 1, ZHANG Ying 4, NIE Xiang ting 4(1 Institute for Molecular Biology of Medicine, Nanjing Medical University, Nanjing 210029,China;2 Nanjing Agricultural University ,Nanjing 210095,China;3 Institute of Animal Science and Veterinary Medicine, Jiangsu Academy of Agricultural Science, Nanjing 210014,China;4 College of Animal Technology, Shanxi Agricultural University,Taigu 030801, China)  
To design and identify the CpG oligonucleotide (ODN) with immunostimulatory activities in porcine peripheral blood mononuclear cells, we had screened 38 CpG ODN The stimulatory index was determined by 3 H TdR incorporation assay and the levels of IFN γ and IL 2 in the culture supernatants were detected with the appropriate ELISA testing kids(swIFN γ kid and swIL 2 kid respectively) The results showed that CpG ODN could stimulate the proliferation of porcine PBMC and the secretion of IFN γ, with little secretion of IL 2 Among the ODN, ODN 2006 that contained 3 GTCGTT motifs could trigger the proliferation of porcine PBMC most strongly with a stimulatory index of 6 2,but only induce a low level of IFN γ On the contrary, ODN D19 with a mixed backbone of phosphodiester and phosphorothioate and a poly G tail, showed moderately immunostimulatory activities on porcine PBMC,but it could induce PBMC to release large amount of IFN γ It concludes that ODN 2006 as well as ODN D19 have different immunostimulatory activities on porcine PBMC
【Fund】: 国家高技术研究发展计划 ( 863计划 ) (No 2 0 0 1AA2 43 0 71)
【CateGory Index】: R392.11
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