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《》 2003-04
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Establishment and Applications of a Sandwich ELISA for Detection of Soluble Leukocyte Associated Immunoglubulin-Like Receptor 1

XUE Jiang nan, OUYANG Wei ming, JIA Wei, XIE Xin,LIU Jing mei, NING Shuang fei, Hu Yi,SONG Chao jun JIN Bo quan ( Department of Immunology, Fourth Military Medical University, Xi'an 710032,China )  
Sandwich ELISA for detection of soluble leukocyte associated immunoglubulin like receptor 1(sLAIR 1) was established which may be useful investigating the relation of sLAIR 1 with some diseases.The extracellular region epitopes recognized by anti LAIR 1 mAb were identified by Biosensor and two mAb recognizing the distinct epitopes were employed to establish the sandwich ELISA for detection of sLAIR 1 with a detection limit of 1 5 μg/L The sLAIR 1 was detected both in sera from 24 healthy individuals (6 2±3 3)μg/L, and the sera from 62 patients with hemorrhagic fever with renal syndrome(HFRS) (47 2±35 9)μg/L by using this method In addition, sLAIR 1 was also detected in the culture supernants of PBMC stimulated by PMA, PHA, LPS or anti CD3 mAb sLAIR 1 level found in patients with HFRS was higher than those of the healthy individuals This method may provide a new approach to investigate the immuno regulatory role of LAIR 1 in clinical immunity
【Fund】: 国家重点基础研究发展规划资助项目 ( 2 0 0 1CB5 10 0 0 4)
【CateGory Index】: R446.61
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【Citations】
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1 OU YANG Wei ming, JIN Bo quan,ZHANG Yun,LIU Xue song, LI Qi, XIA Hai binDepartment of Immunology, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China;The inhibitory effect of 9.1C3 molecule on cytotoxicity mediated by human natural killer cells and T cells[J];JOURNAL OF CELLULAR AND MOLECULAR IMMUNOLOGY;2000-02
2 XUE Jiang nan, OUYANG Wei ming, JIA Wei, ZHANG Xin hai, XIE Xin, CAO Yun xin, NING Shuang fei, LI Qi and JIN Bo quan Department of Immunology, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China;Eukaryotic expression and purification of 9.1C3/LAIR-1 and its preparation of monoclonal antibody[J];Journal of Cellular and Molecular Immunology;2002-06
3 LIU Jingmei, LIU Xuesong, OUYANG Weiming, ZHU Yong, LI Qi, CAO Yunxin, JIN Boquan. Department of Immunology, Fourth Military Medical University, Xi′an 710032, China;The study of T_H1/T_H2 and Tc1/Tc2 balance and cytokine changing in the pathogenesis of hemorrhagic fever with renal syndrome[J];Chinese Journal of Microbiology and Immunology;2002-06
【Co-citations】
Chinese Journal Full-text Database 7 Hits
1 XIE Xin,CAO Yun-xin,WANG Wei,XUE Jiang-nan,OUYANG Wei-ming,WANG Chun-yan,JIN Bo-quan(Department of Immunology, Fourth Military Medical University, Xi'an 710032, China);Preparation and Identification of Monoclonal Antibodies Against Leukocyte-Associated Immunoglobulin-Like Receptor-2 (LAIR-2) and Establishment of Sandwich ELISA for Detecting LAIR-2[J];;2004-01
2 CHEN Baoguo, LI Boli, ZHU Min, HE Zebao. Central Laboratory of Taizhou Hospital, Wenzhou Medical College, Zhejiang Linhai 317000, China;Dynamic alternation of lymphocyte subsets in patients with hemorrhagic fever with renal syndrome[J];Shanghai Journal of Medical Laboratory Sciences;2005-04
3 OU-YANG Wei-ming, LI Qi, LIU Xue-song, ZHANG Yun, JIA Wei, LI De-min, XIA Hai-bin, JIN Bo-quan Department of Immunology, Fourth Military Medical University, Xi'an,710032, Shaanxi Province, China;Study on the effects and its mechanism of 9.1C3 on CD2 or CD3 mediated cytotoxicities[J];Journal of Cellular and Molecular Immunology;2002-02
4 XUE Jiang nan, OUYANG Wei ming, JIA Wei, ZHANG Xin hai, XIE Xin, CAO Yun xin, NING Shuang fei, LI Qi and JIN Bo quan Department of Immunology, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China;Eukaryotic expression and purification of 9.1C3/LAIR-1 and its preparation of monoclonal antibody[J];Journal of Cellular and Molecular Immunology;2002-06
5 XIE Xin, OU YANG Wei ming, XUE Jiang nan, ZHANG Xin hai, LIU Fei, ZHUANG Ran, JIN Bo quan Department of Immunology, Fourth Military Medical University, Xi’an 710032, China;Cloning, prokaryotic expression of LAIR and identification of their immunological reactivities[J];Journal of Cellular and Molecular Immunology;2003-02
6 CHEN Yong, L He zuo, HU Jian guo, LI Bai qing Department of Immunology, Bengbu Medical College, Bengbu 233003, China;Characterization of Mycobacterium tuberculosis polypeptide antigen capable of stimulating the proliferation of human γδT cells[J];Journal of Cellular and Molecular Immunology;2003-02
7 OU YANG Wei Ming *,WANG Hong Fang,WANG Yun et al.Department of Membrane Biology,Institute of Zoology,Chinese Academy Science,Beijing 100080;*Department of Immunology,Fourth Military Medical University,Xi′an 710032;Effect of anti-CD226 mAb and anti-9.1C3 mAb on the cytotoxicity of NK-92 cell line[J];Chinese Journal of Immunology;2002-11
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4 Fu Yuyang,Hiaoshun,Chen Jianlin,et al.Organ Transplantation Center,the First Affilliated Hospital,Sun Yat sen University of Medical Sciences,Guang Zhou 510080;EXPRESSION OF SICAM-1 IN RAT BILE AND ITS DIAGNOSTIC VALUEIN ACUTE LIVER REJECTION[J];Journal of Hepatobiliary Surgery;2001-01
5 Li Zhouli, Shi Bingyi, Cai Ming et al. 309 Hospital of PLA, Beijing 100091;EXPRESSION OF AN ACTIVATED T CELL ANTIGEN p140 IN ALLOGRAFT RENAL TRANSPLANTATION[J];Medical Journal of Chinese People's Liberation Army;2002-10
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7 XIE Xin,CAO Yun-xin,WANG Wei,XUE Jiang-nan,OUYANG Wei-ming,WANG Chun-yan,JIN Bo-quan(Department of Immunology, Fourth Military Medical University, Xi'an 710032, China);Preparation and Identification of Monoclonal Antibodies Against Leukocyte-Associated Immunoglobulin-Like Receptor-2 (LAIR-2) and Establishment of Sandwich ELISA for Detecting LAIR-2[J];;2004-01
8 LI Zhouli,SHI Bingyi,CAI Ming,QIAN Yeyong,MO Chunbai,JIA Wei,JIN Boquan Central of Organ Transplatation , The No 309 Hospital of People Liberation Army , Beijing 100091 The Department of Immunology ,The Forth Military Medical Univercity ,Xi an 710032;Changes of serum soluble and membranous platelet - T cell activation antigen 1 in renal transplant recipients with acute rejection[J];Chinese Journal of Nephrology, Dialysis & Transplantation;2003-03
9 OU YANG Wei ming, JIN Bo quan,ZHANG Yun,LIU Xue song, LI Qi, XIA Hai binDepartment of Immunology, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China;The inhibitory effect of 9.1C3 molecule on cytotoxicity mediated by human natural killer cells and T cells[J];JOURNAL OF CELLULAR AND MOLECULAR IMMUNOLOGY;2000-02
10 DONG Guang long1,WANG Wei zhong1,WU Guo sheng1, SONG Wei liang1, LI Qi2, JIN Bo quan21Department of Gastrointestinal Surgery, Xijing Hospital, 2Department of Immunology, Fourth Military Medical University, Xi'an, 710032, Shaanxi Province, China;The clinical significances of Th1 and Th2-as-sociated cytokines in living-related small bowel transplantation[J];JOURNAL OF CELLULAR AND MOLECULAR IMMUNOLOGY;2000-04
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 XUE Song-guo,FAN Li-an;Construction and sequencing of eukaryotic expression vector of human LAIR-1-IgFc fusion protein gene[J];Immunological Journal;2006-03
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 OU YANG Wei ming, JIN Bo quan,ZHANG Yun,LIU Xue song, LI Qi, XIA Hai binDepartment of Immunology, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China;The inhibitory effect of 9.1C3 molecule on cytotoxicity mediated by human natural killer cells and T cells[J];JOURNAL OF CELLULAR AND MOLECULAR IMMUNOLOGY;2000-02
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