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《Current Immunology》 2007-01
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Selection and preliminary identification of hepatocarcinoma-binding and internalizing peptide

LI Jing,WANG Lei,HAN Hong-hui,DU Bing,QIAN Min(Laboratory of Immunology,School of Life Science,East China Normal University,Shanghai 200062,China)  
Phage display peptide libraries had been successfully used to screen hepatocarcinoma-binding and internalizing peptides.With great potential to be used in targeted drug therapy for liver cancer.In the present study the phage display 12-mer peptide library was screened with liver cancer cell line BEL-7404,and after three rounds of biopanning,20 positive clones were picked up and sequenced.Their affinity to BEL-7404 was identified by cell-based ELISA.Then,the clone LJ08 were chosen for further identification by immunofluorescence and FACS.It was demonstrated that the phage titers were significantly improved after each round of biopanning.The results of cell-based ELISA indicated that LJ08 had the highest affinity to BEL-7404.Besides,LJ08 were identified efficiently endocytosed into BEL-7404 cells,but not the normal cell line as demonstrated by immunofluorescence and FACS.It is feasible to screen and isolate targeted peptides that bind specifically to and internalizing into BEL-7404 cells using phage display peptide libraries.These results may lay a foundation for LJ08 to become a drug carrier used for the targeted therapy of hepatocellular carcinoma.
【Fund】: 上海市科技发展基金重点资助项目(04JC14033、00JC14049);; 国家自然科学基金资助项目(30440039)
【CateGory Index】: R735.7
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