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《Current Immunology》 2007-01
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The in vitro and in vivo chemotactic effect on immune cells by hMIP-1β gene transfected murine colorectal adenocarcinoma CT26 cells

LUO Xiao-ling,XIE Yu-an,KUANG Zhi-peng,WU Ji-ning,LIANG An-min(Department of Experiment Study,Affiliated Tumor Hospital of Guangxi Medicial University,Nanning 533021,China)  
This study was to investigate the changes of biological characters and tumorigenicity and the chemotactic activities to immune cells of tumor cells transfected with human macrophage inflammatory protein-1 beta(hMIP-1β) gene.Murine colorectal adenocarcinoma CT26 cells were transfected with a recombinant adenovirus carrying the hMIP-1β gene(AdhMIP-1β).Then the gene transfected was tested by X-gal method and hMIP-1β level in supernatants of hMIP-1β transfected CT26 cells was assayed by ELISA.The chemotactic activity for CD4~(+) T cells,CD8~(+) T cells,NK cells and immature dendritic cells(imDC) were assayed by a transwell chamber,and change of growth characterization and in vivo tumorigenicity of hMIP-1β gene-transfected CT26 cells were also investigated.The results showed that hMIP1β gene could be transfected into CT26 cells by AdhMIP-1β efficienctly.The level of hMIP-1β in the culture supernatant of hMIP-1β gene-transfected CT26 cells was enhanced and the supernatant displayed marked chemotactic activities to CD4~(+) T cells,CD8~(+) T cells,NK cells and imDC compared with LacZ gene-transfected CT26 cells and control.When the hMIP-1β gene-transfected CT26 cells were subcutaneously inoculated to BALB/c mice,tumorigencity was delayed and suppressed,and overt necrosis and lymphocytes infiltration were observed in tumor tissue but not in those inoculated with LacZ gene-transfected CT26 cells or parental CT26 cells.These findings implicate that hMIP-1β gene could be transfected into CT26 cells and be expressed by AdhMIP-1β with high efficiency,and hMIP-1β gene-transfected CT26 cells exhibited decreased tumorigenicity and could induce antitumor immunity.The data provided a basis for further investigation on tumor vaccine transfected with hMIP-1β gene.
【Fund】: 广西科技厅青年基金资助项目(桂科青0447049)
【CateGory Index】: R735.3
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