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《Current Immunology》 2007-01
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The apoptosis-inducing effects of a novel synthetic nucleoside 2'-O-meth-ylguanosine(P6) on HeLa cell

HUANG Yi~1,NIU Xiu-long~2,ZHANG Hao~1(1.Tianjin Medical University Department of Immunology,Tianjin 300070,China)  
To investigate the apoptosis-inducing effect of a novel synthetic nucleotide—2'-O-methylgranosine(P6) on human cervical carcinoma cell line HeLa cell in vitro,in order to determine whther it has the same antitumor activity as the natural apoptosis-inducing nucleotide(AIN),the proliferation inhibition effect of P6 was evaluated by means of microscopical examination,MTT assay and flow cytometery analysis,and its apoptosis-inducing effect was determined by DNA fragmentation detection,flow cytometry,RT-PCR analysis and chemiluminescence assay.The results showed that the synthetic compound P6 could inhibit the proliferation of HeLa cells by arresting these cells in the S-phase of cell cycle and by down-regulating the transcription of the anti-apoptotic Bcl-2 gene,it could induce apoptosis of HeLa cells.and promote the activation of Caspase-3.All these results suggest that the synthetic nucleotide P6 has the potential attempted to be used as an anti-tumor agent in the future.
【CateGory Index】: R730.5
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