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《Current Immunology》 2009-01
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Effect of anisomycin on the biologic behavior of Jurkat T cells

PAN Shan,XING Fei-yue(Institute of Tissue Transplanation and Immunology,Jinan University,Guangzhou 510632,China)  
ion: To study effect of anisomycin on the activation,proliferation,cell cycle and apoptosis of Jurkat T cells.The expression level of CD69 and CD25 on the surface of Jurkat T cells stimulated by phorbol-12-myristate-13-acetate(PMA) and ionomycin(Ion) was evaluated by fluorescin-conjugated monoclonal antibody double labeling technique and the proliferation of Jurkat T cells was analyzed by MTT method and inverted microscope.Meanwhile,the Jurkat T cell cycle was analyzed by PI staining and flow cytometry and the apoptosis of Jurkat T cells was analyzed by Annexin V-FITC and PI staining.It was found that the CD69 and CD25 expressing level of Jurkat T cells that were activated by PMA and ionomycin was decreased along with the increase of anisomycin concentration,and the colony formation of Jurkat T cells decreased along with the increase of anisomycin concentration.The highest inhibitory rate of Jurkat T cells was 74.29%,and Jurkat T cell cycle was inhibited obviously by anisomycin before phase of G0/G1.The apoptosis of Jurkat T cells was promoted by the dose of 40.0 ~160.0 ng/ml anisomycin.It is obvious that anisomycin can inhibit the activation,proliferation and cell cycle entry of Jurkat T cells,and promote the apoptosis of Jurkat T cells.
【Fund】: 国家自然科学基金资助项目(30471635);; 广东省自然科学基金资助项目(04010451 5006033)
【CateGory Index】: R392.12
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