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《Current Immunology》 2009-01
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Effects of Forskolin on activation and proliferation of the murine T lymphocytes in vitro

SONG Bing,ZENG Yao-ying,HUANG Xiu-yan,LI Lin,TENG Fei,YANG Zhi,YAO Man-lin(Institute of Tissue Transplantation and Immunology,Jinan University,Guangzhou 510632,China)  
Investigating the effects of Forskolin on activation and proliferation of CD3+T lymphocytes stimulated by ConA in vitro,and the expression of IFN-γ,IL-2,IL-4,IL-10 and TNF-α in these cells in order to elucidate the mechanisms of its immunosuppressive mechanism.Signal cell suspensions were prepared from murine lymph nodes and treated by different concentrations of Forskolin.Fluorescence conjugated monoclonal antibodies and flow cytometry were used to detect the expression of CD25 and CD71 activated by ConA.The cytokines from the cultivated supernatant stimulated by ConA were measured by LuminexTM 100,and the proliferation index of mouse T lymphocytes was analyzed by CFDA-SE staining.The experiment results showed that Forskolin(10-7,10-6,10-5 mol/L) could inhibit the expression rate and mean fluorescence intensity(MFI) of CD25 and CD71 on CD3+T lymphocytes in a dose-dependent manner.Forskolin also could inhibit the level of IL-10,IL-2,TNF-α and T lymphocyte proliferation index stimulated by ConA in a dose-dependent manner while increase the level of IFN-γ but no effects on the production of IL-4.It is evident from the above observation that Forskolin can inhibit the activation and proliferation of T lymphocyte by changing the distribution of cytokines and become a potential immunosupressive agents.
【Fund】: 国家重大基础研究资助项目(2006CB504200、2004CB720100);; 广东省基金资助项目(2006B36030016);; 广州市科技局科技攻关重点资助项目(2006Z-E0091);; 生物化学与分子生物学广东省重点学科资助项目
【CateGory Index】: R392.12
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3 SONG Bing,ZENG Yaoying,HUNAG Xiuyan,LI Lin,YANG Zhi,TENG Fei,YAO Manlin Institute of Tissue Transplantation and Immunology,Jinan University,Guangzhou 510632,China;Effects of Forskolin on the activity of the murine T lymphocytes stimulated by phorbol in vitro[J];Immunological Journal;2009-02
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9 SONG Bing,ZENG Yao-ying,HUANG Xiu-yan,LI Lin,TENG Fei,YANG Zhi,YAO Man-lin(Institute of Tissue Transplantation and Immunology,Jinan University,Guangzhou 510632,China);Effects of Forskolin on Proliferation Cell-cycle Distribution and Activation of the Murine T Lymphocytes in vitro[J];Journal of Chinese Medicinal Materials;2008-07
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China Proceedings of conference Full-text Database 1 Hits
1 Jing-Fang Wang~2,Dong-Qing Wei~(1**) (1.College of Life Science and Biotechnology,Shanghai Jiaotong University,Shanghai 200240,China;2.Bioinformatics Center,Key Lab of Systems Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China);Drug candidates from traditional Chinese medicines[A];[C];2008
Chinese Journal Full-text Database 4 Hits
1 ZHENG Xiaoguang , SHEN Xinyuan, YANG Qing (State Key Laberatory for Modification of Fiber and Polymer Materials,China Textile University, Shanghai, China, 200051);The Application of Chitin and its Derivation in Health Field[J];JOURNAL OF HENAN NORMAL UNIVERSITY(NATURAL SCIENCE);1999-03
2 Qiao Yan,Wang Aiqin  (State Key Laboratory of Solid Lubrication,Lanzhou Institute of Chemical and Physics,Chinese Academy of Sciences,Lanzhou 730000);A convenient method for the synthesis of N-acetyl-D-glucosamine[J];Chemical Reagents;2002-03
3 YAO Man-lin,ZENG Yao-ying,HUANG Xiu-yan,ZHOU Jian-guo,LIANG Zhao-duan,YANG Zhi(Institute of Tissue Transplantation and Immunology,College of Life Science and Technology,Jinan University,Guangzhou 510632,China);Effect of minocycline on the in vitro activation and proliferation of murine T lymphocytes[J];Current Immunology;2008-02
4 HAN Bao-qin, LIU Chen-sheng, WANG Xu-min, HE Jun, LIU Chen-guang, LIU Wan-shun (College of Marine Life Sciences, Ocean University of Qingdao, Qingdao 266003);THE EFFECTS OF CM-CHITOSAN, GLUCOSAMINE AND N-ACETYL GLUCOSAMINE ON THE GROWTH AND INSULIN SECRETION OF ISLET CELL[J];Chinese Journal of Biomedical Engineering;2004-02
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 NIU Jia-feng, ZHANG Cai *, ZHANG Jian-hua, XU Xiao-qun, ZHANG Wei-dong, WANG Jun-fu, GAO Chun-yi Institute of Basic Medicine, Shandong Academy of Medical Sciences, Key Laboratory for Tumor Immunity and Genetic Engineering of Shandong Province, Key Laboratory for Modern Medicine and Technology of Shandong Province, Jinan 250062, China;Up-regulation of IFN-α on expression of MICA in the cervical carcinoma cell lines[J];Chinese Journal of Cellular and Molecular Immunology;2006-05
2 Zhang Cai Tian Zhigang Wei Haiming et al Shandong Cancer Biotherapy Center, Shandong Academy of Medical Science, Jinan;The Expression of Non-Classical HLA Class Ⅰ Molecules (HLA-G and HLA-E) in Human Tumor Cell Lines and the Regulatory Effect of IFN-γ[J];Chinese Journal of Clinical Oncology;2001-04
3 ZHANG Cai, TIAN Zhigang, HOU Guihua, et al. Shandong Cancer Biotherapy Center, Shandong Academy of Medical Sciences, Jinan 250062, China;Influence of HLA class Ⅰ molecules expression on tumor cell resistance to NK lysis and the IFN-γ regulatory effect[J];Chinese Journal of Oncology;2001-05
4 ZHANG Cai *, TIAN Zhi gang, ZHANG Jian, FENG Jin bo, ZHANG Jian hua, XU Xiao qun. *Institute of Immunology,School of Life Sciences, University of Science and Technology of China, Hefei 230027,China;The negative regulatory effect of IFN-γ on cognitive function of human natural killer cells[J];Chinese Journal of Oncology;2004-06
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