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《Current Immunology》 2011-01
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The costimulatory effect of mouse B7-H3 gene in the activation of T cells

SUN Jing1,2,YAN Ru-hong1,3,GU Wen-chao1,FU Feng-qing1,ZHANG Guang-bo1,CHEN Xu-qin1,ZHOU Ying-hui1,ZHANG Xue-guang1(1.Institute of Biotechnology,Soochow University,2.Suzhou Health Technology College,3.Clinical Diagnosis Laboratory of the Second Affiliated Hospital,Soochow University,Suzhou 215007,China)  
The biological function of mouse B7-H3 was still not identified yet.This paper was aimed to generate an engineered CHO cell line harboring mouse B7-H3 and to investigate its biological function and effects on T lymphocyte activation.The genes coding mouse B7-H3 were obtained from mouse liver cDNA by RT-PCR and then the target gene fragment was inserted into eukaryotic vector pIRES2-EGFP after being digested with EcoR Ⅰ and Bgl Ⅱ to construct the recombinant vector pIRES2-EGFP/B7-H3.This recombinant vector was transfected into CHO cells with LipfectAMINETM 2000 and followed by G418 selection.RT-PCR,Western blotting and flow cytometry(FCM) were used to detect the expression of B7-H3.Effects of the transfected cells on T cells proliferation and cytokine production in vitro was studied by methods of CCK8 and ELISA.In these ways,a stable cell line constitutively expressing the mouse B7-H3 was established successfully,and the mouse B7-H3 could obviously promote the proliferation of T cells in and secretion of IL-2 and IFN-γ in vitro.This effect could be partly blocked by mAb.It is evident that the engineered mouse B7-H3/CHO cell line provides a gain of function cell model for the further understanding of the biological role of B7-H3.
【Fund】: 国家自然科学基金资助项目(31040022、30771958);; 苏州市社会发展基金资助项目(ss08016)
【CateGory Index】: R392.12
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 Junfa Xu, Baojun Huang, Ping Xiong, Wei Feng, Yong Xu, Min Fang, Fang Zheng and Feili Gong Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China; Department of Clinical Immunology, Guangdong Medical College, Zhanjiang, Guangdong 524023, China; Department of Immunology, Anhui Medical University, Hefei, Anhui 230032, China;Soluble Mouse B7-H3 Down-Regulates Dendritic Cell Stimulatory Capacity to Allogenic T Cell Proliferation and Production of IL-2 and IFN-γ[J];中国免疫学杂志(英文版);2006-03
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【Co-citations】
Chinese Journal Full-text Database 7 Hits
1 RAO Ai-hua~3,ZHOU Ying-hui~(1,2),CHEN Yong-jing~(1,2),MA Zheng-yu~3,ZHANG Xue-guang~(1,2)(1.Biotechnology Institute of Soochow University;2.Key laboratory of Clinic Immunology of Jiangsu Province;3.Biochemistry Department of Soochow University,Suzhou 215007,China);Preparation of a novel functional mouse anti-human 2IgB7-H3 antibody and primary study of its biological characteristics.[J];Current Immunology;2006-04
2 WANG Xue-feng~(1,2),CHEN Yong-jing~(1),WANG Qin~(1),DAI Qun~(1),YANG Ke-fang~(1),ZHANG Xue-guang~(1)(1.Institute of Medical Biotechnology,Soochow University;2.School of Life Science,Soochow University;3.Key Laboratory of Stem Cell of Jiangsu Province,Suzhou 215007,China);Preparation and biological identification of a functional monoclonal antibody,mouse anti-human B and T lymphocyte attenuator[J];Current Immunology;2007-01
3 MA Zhen-yu2*,ZHOU Ying-hui2*,CHEN Yong-jing1,FEI Min1,MA Zhen-ni2,WANG Qin1,WANG Xue-feng1,MA Hong-bing1,XIE Fang1,ZHANG Xue-guang1(1.Institute of Medical Biotechnology;2.Insititute of Biochemistry Engineering,Soochow University,Suzhou 215007,China);Expression of 4IgB7-H3-Fc fusion protein and its biological activity[J];Current Immunology;2007-02
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5 ZHU Yi-bei1,2,ZHANG Guang-bo2,ZHOU Ying-hui1,LI Wen-xiang2,HU Yi-zhou2,ZHANG Xue-guang1,2(1.The Biotechnology Institute of Soochow University,Suzhou 215007,China;2.Key Laboratory of Medicine and Clinical Immunology of Jiangsu Province,the First Affiliated Hospital to Soochow University,Suzhou 215006,China);Effect of cross-linking of the monoclonal antibody against B7-H3 molecule on the in votro biological functions of the peripheral blood monocyte derived dendritic cells[J];Current Immunology;2008-02
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Chinese Journal Full-text Database 1 Hits
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