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《Current Immunology》 2011-01
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Establishment of amplified luminescent proximity homogeneous immunoassay to detect neuron specific enolase in human serum

HE An,DONG Zhi-ning,LIU Tian-cai,ZOU Li-ping,LIN Guan-feng,LI Ming,WU Ying-song(School of Biotechnology,Southern Medical University,Guangzhou 510515,China)  
To establish an amplified luminescent proximity homogeneous immunoassay(AlphaLISA) to detect neuron specific enolase(NSE) in human serum,two anti-NSE monoclonal antibodies were used,in which one was coated on AlphaLISA acceptor beads and the other was biotinylated.This sandwich kit also contained Donor beads covalently coated with streptavidin.The optimal test conditions and analytical performance of the method were then evaluated.The sensitivity of AlphaLISA for NSE was found to be 0.149 ng/ml,and the linear range of the developed kit was 0.149~600 ng/ml.The intra-and inter-assay coefficient of variation(CV) were 3.7~4.3% and 3.9~5.2%,respectively.There was no cross-reaction to CYFRA21-1 and NNE compared with the commercially available elecsys NSE kit(Roche),the correlation coefficient of CEA-AlphaLISA was 0.979.NSE AlphaLISA appears to be a new immunoassay which have several advantages including high sensitivity,specificity,a wide working range,rapidity and convenience of testing and to be suitable for routine clinical detection of NSE in human serum.
【Fund】: 国家科技重大专项(2009ZX10004-706)
【CateGory Index】: R392-33
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