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《Current Immunology》 2011-01
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Heat-killed Candida albicans stimulates human peripheral blood mononuclear cells to produce IL-12 and IFN-γ partly through Dectin-1

HU Zhi-de,HUANG Yuan-lan,SUN Yi,CHEN Sun-xiao,DENG An-mei,ZHONG Ren-qian(Department of Laboratory Diagnostics,Changzheng Hospital,Second Military Medical University,Clinical Immunology Center of People's Liberation Army,Shanghai 200003,China)  
To investigate the role of Dectin-1 in heat-killed Candida albicans-inducing IL-12 and IFN-γ productiong in peripheral blood mononuclear cells(PBMCs),human PBMCs were firstly pre-incubated with laminarin,a Dectin-1 specific blocker,for one hour and then stimulated with heat-killed Candida albicans for 4 days.In addition,Zymosan,a Dectin-1 activating agent,was used to stimulate PBMC at different dose,and the levels of IL-12 and IFN-γ were measured in the cultural supernatants by ELISA assay.It was demonstrated that laminarin partially decreased the efficiency of heat-killed Candida albicans to induce IL-12 and IFN-γ production in PBMCs.Zymosan could stimulate production of IL-12 and IFN-γ in a dose-dependent manner.From these observations it suggests that the production of IL-12 and IFN-γ in PBMCs by Candida albicans may be partly mediated through Dectin-1.
【Fund】: 国家自然科学基金资助项目(81072479、30772017、30972730);; 上海市科委基金资助项目(09JC1405400、08QH14001)
【CateGory Index】: R392.12
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