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《Current Immunology》 2011-01
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Evaluation on the detection of anti-nuclear antibody and anti-soluble nuclearantigen antibody for the diagnosis of autoimmune disease

LU Hui-qi,LI Chang,YE Wei-min,YANG Jie,HE Ming-jun,HAN Huan-xing(Department of Laboratory Medicine,Changzheng Hospital,Second Military Medical Univercity,Shanghai 200003,China)  
To evaluating the sensitivity and correlation on anti-nuclear antibody(ANA) and anti-soluble nuclear antigen antibody(ENA) in the diagnosis of autoimmune disease(AID),the results of auto-antibody detection in serum were retrospectively analyzed for patients visited at Changzheng hospital from Jan,2007 to Oct,2009,among which 224 cases were patients with AID and 325 cases were patients with non-autoimmune diseases.For all these patients,the indirect immunofluorescene(IIF) assay based on the cultured human epidermoid carcinoma cells(Hep-2 cell/liver and immunoblot assay were used to detect ANA and ENA respectively.It was demonstrated that 4 different patterns were formed by using these two kinds of detection methods,i.e.ANA+/ENA+、ANA-/ENA-、ANA+/ENA-and ANA-/ENA+ respectively.Positive rate of ANA and ENA(63.4% vs 58.5%) in AID was significantly higher than that of non-AID(16.9% vs 25.2%).Expect in with MCTD and SLE,there were no correlation between ANA and ENA.The ANA-IIF assay appears to be a time-consuming procedure and difficult to standardize owing to subjective interpretation of results.Acting as a screening experimental method,the sensitivity of ANA is higher than that of ENA.Nevertheless.the combined use of both methods would increase the sensitivity and reliability of testing.
【CateGory Index】: R593.2
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