Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Current Immunology》 2011-01
Add to Favorite Get Latest Update

The influence of the mouse recombinant adenovirus vector Ad-mIL-10 and its transfected dendritic cells on the airway inflammation in asthmatic mouse model

ZHANG Wei,CHEN Chuan-hui,XU Fei,LIN Yao-guang(Department of Respiratory Medicine,The First Affiliated Hospital of Nanchang University,Nanchang 330006,China)  
To study the influence of the mouse recombinant adenovirus vector Ad-mIL-10 and its transfected dendritic cells(DCs) on the mouse airway inflammation in allergic asthma and to search for the related pathogenesis,this vector was constructed by using gene engineering methods and transfected to,DCs derived from bone marrow of mice.Mouse asthmatic model was established by intraperitoneal injection of ovalbumin(OVA) for sensitization and followed by OVA inhalation.Ad-mIL-10 or the DCs transfected by Ad-mIL-10 was adoptively transferred to mice after the first OVA intraperitoneal injection.The changes in the proportions of CD3+IL-10+IL-4-Tr cells and CD3+IL-10+IL-4+ Th2 cells in blood and brochoalveolar lavage fluid(BALF) were observed by flow cytometry,and the levels of IFN-γ,IL-4 and IL-10 were tested by ELISA to observe the quantity and function alterations of Th1 and Th2 cells in asthma.The experimental results showed that no significant influence of Ad-mIL-10 on the quantity and function of Tr cells,Th1 and Th2 cells in lungs of the mouse asthmatic model was observed,but it showed certain inhibitory effect on the function of blood Th2 cells.DC transfected by Ad-mIL-10 could induce the increase of IL-10+IL-4-Tr cells and the decrease of IL4+IL-10+Th2 cells and suppress the Th2 function of mouse asthmatic model,but have no impact on the Th1 function.From these observations,it is evident that either Ad-mIL-10 or DC transfected by Ad-mIL-10 induce immunologic tolerance,but simple application of Ad-mIL-10 to produce high concentration of IL-10 is unable to induce the development of Tr cells and the suppression of Th2 cell activation.However,the Ad-mIL-10 transfected DCs can induce the naive T cells to differentiate into Tr cells,and suppress the quantity and function of Th2 cells.
【Fund】: “十一五”国家科技支撑计划资助项目(2008BAI68B00)
【CateGory Index】: R562.25
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 3 Hits
1 ZHAO Li-hua,REN Ya-na,NIE Xiao-xuan,YANG Jie,GAO Li,LIU Yuan-yuan,GAO Fang,FAN Hua-hua(Shanghai Blood Center Shanghai 200051,China);In vitro induction of immune tolerance by exosomes secreted from regulatory dendritic cells[J];Current Immunology;2008-01
2 CHEN Yan,FU Zhou,CHEN Kun-hua,YANG Xi-qiang,LIU En-mei,WANG Li-jia,LI Xin(Department of Immunology,Children's Hospital,Chongqing University of Medical Science,Chongqing 400014,China);Human peripheral blood dendritic cells of patients with asthma participate the Th1/Th2 balance shift[J];Current Immunology;2008-03
3 Xu Fei,Chen Chuan-hui,Lin Yao-guang,Zhang Wei Department of Respiratory Medicine,First Affiliated Hospital of Nanchang University,Nanchang 330006,Jiangxi Province,China;Construction of mouse interleukin-10 recombinant adenovirus vector and gene modification for dendritic cells[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2010-05
【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 REN Ya-na,YANG Jie,LIU Yan,et al.Blood Engineering Laboratory,Shanghai Blood Center,Shanghai 200051;The Primary Study on the Acute GVHD Suppression by Exosomes Derived from Regulatory DC[J];Journal of Clinical Transfusion and Laboratory Medicine;2010-01
2 ZHOU Xiao-rong1,WANG Xiao-ying1,TANG Wei2,SUN Xiao-lei1,WU Qiong 1,HAN Yu1(1. Immunology Department of Medical College,Nantong University,Nantong 226001,China;2.Department of Infectious Disease of Affiliated Hospital,Nantong University,Nantong 226001,China);Influence of dendritic cells on Th1/Th2 differentiation in patients with chronic hepatitis B[J];Current Immunology;2007-05
3 XU Wen1,LI Xin-qun2(1.Department of Microbiology and Immunology,Wenzou Medical College,Wenzhou 325035,China;2.First Hopital of Sanming,Sanming 365000,China);Polyporus polysaccharide stimulates and induces the functional maturation of dendritic cells derived from murine bone marrow[J];Current Immunology;2008-04
4 MEI Ai-nong,WANG Jue,YANG Jin,ZHANG Su-ming(Department of Neurology,Fujian Provincial Hospital,Fuzhou 350001,China);Differentiation of ES-D3 embryonic stem cells into dendritic cells in vitro[J];Current Immunology;2010-02
5 ZHANG Fang1,SHI Yi1,LI Zi-ling1,HU Bo2,SONG Yong1(1.Department of Respiration Diseases,Nanjing Genaral Hospital of Nanjing Military Command;2.Joint Logistics Department of the Nanjing Military Region,Nanjing 210002,China);Fusion protein mTSLPR-Ig regulates CD11c~+ cells and improve the inflammatory reactions in a mouse model of asthma[J];Current Immunology;2011-01
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 SHI Jun1,HUANG Ya-juan2,WAN Yun1,WANG Min1,IKEDA Kazuma3(1.Hematological Department;2.Department of Gynaecology and Obstetrics,Sixth People's Hospital of Shanghai Jiaotong University,Shanghai 200233,China;3.Second Department of Internal Medicine,Okayama University Hospital,Japan 700-8558);The biological characteristics of cord blood CD123~+ myeloid dendritic cell[J];Current Immunology;2007-05
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved