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《Current Immunology》 2014-01
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Expression of miR-486-5p in hepatocllular cancer and its effect on the growth of HCC cells

HUANG Xin-ping;HOU Jin;SHEN Xiao-yun;LU Hai-juan;LUO Xiao-ling;Research Department,Affiliated Cancer Hospital,Guangxi Medical University;National Key Laboratory of Medical Immunology and Institute of Immunology,Second Military Medical University;  
This study aims to investigate the expression of miR-486-5p in hepatocellular carcinoma(HCC) and to explore its role in the growth of HCC cells.Firstly,the expression of miR-486-5p in 40 samples of HCC tissue and their matched controls as well as in a group of HCC cell lines(QGY-7701,QGY-7703,BEL-7404,SMMC-7721,Huh7,HepG2 and PCL/PRF/5) was tested by qRT-PCR.Secondly,synthetic miR-486-5p mimics or negative control(NC) were respectively transfected into the above mentioned cell lines by liposome method,and their effect on HCC proliferation was tested by CCK8 assay.Last,the role of miR-486-5p on HCC cells cell cycle and apoptosis was detected by FACS.The results showed that:1.Consistent with the result of miRomes MPSS,the expression of miR-486-5p was downregulated in 40 paired HCC tissues and HCC cells compared to that in the match control by qRT-PCR(P 0.05).2.CCK8 assay revealed that miR-486-5p can effectively and steadily inhibit proliferation of all the HCC cells tested(P 0.05).3.FACS technology found that miR-486-5p overexpression could induce HCC cells apoptosis(QGY-7703 cell P 0.001;QGY-7701 cell P 0.01) and blocked cell cycle progression of HCC cells at G1 phage(P 0.01).In conclusion,these results indicate that miR-486-5p is markedly decreased in HCC,and its restoration can inhibit HCC cell proliferation by arresting cell cycle at Glphase and inducing apoptosis.These evidences suggest that miR-486-5p may play important roles in HCC development.
【Fund】: 第二军医大学免疫学研究所暨医学免疫学国家重点实验室开放课题
【CateGory Index】: R735.7
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