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《Current Immunology》 2014-01
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Preparation and characterization of a mouse anti-human HVEM monoclonal antibody

SONG Hua-feng;QI Yuan;HUANG Zi-yi;WU Mei-ying;XU Ping;WANG Xue-feng;ZHANG Xue-guang;School of Biology and Basic Medical Siences,Soochow University;Institute of Medical Biotechnology,Soochow University;The Affiliated Infectious Hospital of Soochow University;  
To generate a monoclonal antibody(mAb) against human herpesvirus entry mediator(HVEM) and to characterize its biological functions,L929/HVEM,a stable transfectant that expresses human HVEM,was used as the antigen to immunize BALB/c mice.After immunization and fusion,a hybridoma which could specifically secrete anti-human HVEM mAb was selected with L929/HVEM as target cells and L929/mock as the negative control.The specificity of the anti-human HVEM mAb generated by the hybridoma was further identified by testing the binding of the mAb to L929/HVEM transfectant and a group of tumor cell lines using flow cytometry(FCM) and Western blot.Moreover,we investigated its effects on T cell proliferation and cytokine secretion in vitro by MTT assay and ELISA analysis.The results showed that a hybridoma secreting anti-human mAb(named 2B11) was successfully prepared which could specifically recognize HVEM expressed by the transfectant L929/HVEM and tumor cell lines and block T cell proliferation as well as cytokine secretion.
【Fund】: 国家自然科学基金重点项目(30930085);; 973课题(2013CB530501);; 苏州市科技发展计划(SS201246;SS201241;SYS201101)
【CateGory Index】: R392.12
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 DAI Qun~1 WANG Xue-feng~(1,2) CHEN Yong-jing~1 WANG Qin~1 ZHOU Yin-hui~(1,3) XIE Fang~(1,3) ZHANG Xue-guang~1 (1.Institute of Biotechnology,Soochow University; 2.School of Life Science,Soochow University; 3.Department of Basic Medicine,Soochow University,Suzhou 215007,China);Preliminary analysis of the regulatory expression and inhibitory function of the human herpesvirus entry mediator HVEM[J];Current Immunology;2007-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHA Zhi-hui,SHI Hui,XIE Jian-jun,et al(Marine and Fishery Research Institute of Zhejiang Ocean University,Marine and Fisheries Research Institute of Zhejiang Province,Zhejiang Province Key Lab of Mariculture and Enhancemen,Zhoushan 316100,China);Establishment of Double Antibody-Sandwich ELISA for Detection of Marine Crab Hematodinium sp.[J];Journal of Zhejiang Ocean University(Natural Science);2011-01
2 JI Yu-hong 1,2 ,QIU Yu-hua 1,WANG Yue-dan 1,JU Song-guang 1,LI Cai-xia 1,FU Jin-xiang 1,QIAN Ji-hu 2,ZHANG Xue-guang 1(1 Department of Immunology,Soochow University,Suzhou 215007;2 Nantong Medical College,Nantong 226001);Preparation of Functional Monoclonal Antibody Against Human IL-11 and Analysis of Its Biological Characteristics[J];Shanghai J.of Immunology;2002-06
3 JU Song-wen, LIU Ling, JU Song-guang, YU Ge-hua, PAN Jian-zhong, QIU Yu-hua, ZHANG Xue-guang ( Biotechnology Research Unit, Soochow University, Suzhou 215007, China );Preparation of Anti-Human CD28 Functional Monoclonal Antibody and Characterization of Its Biological Activities[J];Shanghai J.of Immunology;2003-05
4 RAO Ai-hua~3,ZHOU Ying-hui~(1,2),CHEN Yong-jing~(1,2),MA Zheng-yu~3,ZHANG Xue-guang~(1,2)(1.Biotechnology Institute of Soochow University;2.Key laboratory of Clinic Immunology of Jiangsu Province;3.Biochemistry Department of Soochow University,Suzhou 215007,China);Preparation of a novel functional mouse anti-human 2IgB7-H3 antibody and primary study of its biological characteristics.[J];Current Immunology;2006-04
5 TIAN Xiao-jing ZHOU Chun-gang LI Dao-ming XU Ying YU Ge-hua ZHANG Xue-guang GU Zong-jiang(Department of Immunology,School of Medicine,Soochow University,Suzhou 215007,China);Preparation of anti-human 4-1BB monoclonal antibody and analysis of its biological functions[J];Current Immunology;2007-03
6 HE Na2*,ZHOU Ying-hui1,2*,ZHANG Guang-bo3,CHEN Yong-jing1,WANG Xue-feng1,2,QIU Tian-yu2,GU Wen-chao2,CUI Hao-jie4,ZHANG Xue-guang1,3(1.Institute of Medical Biotechnology,Soochow University,Suzhou 215007,China;2.Department of Biochemistry and Molecular Biology,School of Medicine,Soochow University,Suzhou 215123,China;3.Key Laboratory of Clinic Immunology of Jiangsu Province,No.1 Affiliated Hospital,Soochow University,Suzhou 215006,China;4.Department of Plastic and Burns Surgery,No.1 Affiliated Hospital,Soochow University,Suzhou 215006,China);Preparation of a monoclonal antibody against human 4IgB7-H3 molecule and analysis of its biological characteristics[J];Current Immunology;2009-06
7 HU Zhen-hua,CHEN Yong-jing,WANG Qin,BAI Li-xiong,WANG Xue-feng,ZHANG Xue-guang(Medical Biotechnology Institute of Soochow University,Suzhou 215007,China);Preparation and characterization of a novel monoclonal antibody against human PD-1[J];Current Immunology;2010-01
8 MAO Hui,XIE Fang,CHEN Yong-jing,WANG Qin,ZHANG Xue-guang(Medical Biotechnology Institute of Soochow University,Suzhou 215007,China);Preparation of anti-human OX40 agonist monoclonal antibody and its biological function[J];Current Immunology;2010-03
9 BAI Li-xiong,CHEN Yong-jing,ZHOU Yin,ZHANG Xue-kun,MA Yu,ZHANG Xue-guang(Medical Biotechnology Institute of Soochow University,Suzhou 215007,China);Preparation and characterization of a novel monoclonal antibody against human PD-L2(B7-DC)[J];Current Immunology;2011-04
10 XU Jun-chi,FU Feng-qing,GAO Fei,ZHOU Bin,HU Yu-min,ZHANG Xue-guang(Medical Biotechnology Institute of Soochow University,Suzhou 215007,China);Preparation and biological characterization of a mouse anti-human monoclonal antibody against TLT-2[J];Current Immunology;2011-05
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 WANG Qin 1,SUN Jian-jun 1,SHI Qin 1,CHEN Yong-jing 1,DAI Jun 1,CHEN Jie 1,SU Yong-qian 2,ZHANG Xue-guang 1(1. Institute of Medical Biotechnology,Soochow University,Suzhou 215007,China;2. Tumor Department,Jiangsu People's Hospital,Nanjing 210009,China);Construction of the Transfected Cell Line Expressing the Human OX40L Gene and Its Costimulation to T Cells[J];;2004-02
2 ZHANG Guang-bo, CHEN Yong-jing, JIANG Zhi, YU Ge-hua, YANG Ming-feng, SHI Qin, WANG Qin, LI Wen-xiang, ZHANG Xue-guang(Biotechnology Institute of Soochow Univerisity, Key Laboratory of Clinic Immunology of Jiangsu Province, Suzhou 215007, China);Construction of Human B7-H3 Transfected Cell Line and the Investigation of Its Bioactivity in vitro[J];;2004-06
3 Zhang Yi et al;A Monoclonal Antibody(C_3H_6)Against Human Leukemia Cells and its Characterization[J];;1989-02
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