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《Current Immunology》 2014-01
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Preparation and characterization of a mouse anti-human HVEM monoclonal antibody

SONG Hua-feng;QI Yuan;HUANG Zi-yi;WU Mei-ying;XU Ping;WANG Xue-feng;ZHANG Xue-guang;School of Biology and Basic Medical Siences,Soochow University;Institute of Medical Biotechnology,Soochow University;The Affiliated Infectious Hospital of Soochow University;  
To generate a monoclonal antibody(mAb) against human herpesvirus entry mediator(HVEM) and to characterize its biological functions,L929/HVEM,a stable transfectant that expresses human HVEM,was used as the antigen to immunize BALB/c mice.After immunization and fusion,a hybridoma which could specifically secrete anti-human HVEM mAb was selected with L929/HVEM as target cells and L929/mock as the negative control.The specificity of the anti-human HVEM mAb generated by the hybridoma was further identified by testing the binding of the mAb to L929/HVEM transfectant and a group of tumor cell lines using flow cytometry(FCM) and Western blot.Moreover,we investigated its effects on T cell proliferation and cytokine secretion in vitro by MTT assay and ELISA analysis.The results showed that a hybridoma secreting anti-human mAb(named 2B11) was successfully prepared which could specifically recognize HVEM expressed by the transfectant L929/HVEM and tumor cell lines and block T cell proliferation as well as cytokine secretion.
【Fund】: 国家自然科学基金重点项目(30930085);; 973课题(2013CB530501);; 苏州市科技发展计划(SS201246;SS201241;SYS201101)
【CateGory Index】: R392.12
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