Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Current Immunology》 2014-01
Add to Favorite Get Latest Update

Construction and functional analysis of a mouse secretory TIGIT lentiviral vector

CHEN Xi;GAO Yi;HUANG Min;AIHAITI Aziguli;CHEN Zhong-hua;YV Xue-feng;HE Wen-tao;Department of Endocrinology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology;Institute of Organ Transplantation,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology;Department of Laboratory Medicine,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology;  
Our aims are to construct a lentiviral vector Lenti-TIG-Fc which encodes the extracellular domain of mouse TIGIT and the human IgG-Fc fusion gene,and to verify the functions of the fusion protein TIG-Fc and lay a foundation for the further study in immune regulation.The extracellular domain of mouse TIGIT and human IgG-Fc fusion gene was cloned into plasmid pLOX.Correctly connected cloning TIG-Fc-pLOX was identified and then co-transfected into HEK 293T cells together with pCMVAR8.2 and pMD.G,The supernatant containing lentivirus Lenti-TIG-Fc was harvested.293T cells infected hy the Lenti-TIG-Fc were co-cultured with LPS-induced mature dendritic cells(DCs) from BALB/c mice.IL-10 level secreted by DCs was detected by ELISA.The results showed that TIG—Fc fusion gene was successfully cloned and inserted to lentivirus plasmids.293T cells infected by the Lenti-TIG-Fc consistently secret TIG-Fc fusion protein and could significantly promote secretion of IL-10 by mature DCs.
【Fund】: 国家自然科学基金(81102260);; 中央高校基本科研业务费资助(HUST2012QN190)
【CateGory Index】: R392
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Co-citations】
Chinese Journal Full-text Database 4 Hits
1 ZHAO Li-hua,REN Ya-na,NIE Xiao-xuan,YANG Jie,GAO Li,LIU Yuan-yuan,GAO Fang,FAN Hua-hua(Shanghai Blood Center Shanghai 200051,China);In vitro induction of immune tolerance by exosomes secreted from regulatory dendritic cells[J];Current Immunology;2008-01
2 ZHANG Fang1,SHI Yi1,LI Zi-ling1,HU Bo2,SONG Yong1(1.Department of Respiration Diseases,Nanjing Genaral Hospital of Nanjing Military Command;2.Joint Logistics Department of the Nanjing Military Region,Nanjing 210002,China);Fusion protein of thymic stromal lymphopoietin-immunoglobulin inhibits the in vitro activation on dendritic cells by TSLP[J];Current Immunology;2010-05
3 BAO Jie1,WANG Qian2,ZHENG Lei1,XIONG Shi-long1,QIU Yu-rong1(1.Department of Laboratory Medicine,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China;2.Administration Office,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China);The effect of nuclear factor RelB suppression on the induction of immunological tolerance in vitro of mice bone marrow-derived dendritic cells[J];Current Immunology;2010-06
4 YAO Min-xue;ZHANG Li;ZHI Liang;HU Min-dao;The Second Affiliated Hospital of Kunming Medical University,Department of Hepatobiliary and Pancreatic Ward;;Expression and identification of recombinant adenovirus mediated Macaca mulatta CD40L extracellular period of gene in Macaca mulattaimmature dendritic cells.[J];Current Immunology;2013-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved