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《Current Immunology》 2014-03
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Analysis on angiotensin Ⅱ type 1 receptor agonistic autoantibody in hyperthyroidism rats associated with cardiac hypertrophy and its relationship with microRNA-350

XU Jin-ling;ZHAO Lin-shuang;WANG Min;Department of Endocrinology and Metabolism,Wuhan General Hospital of Guangzhou Command;Institute of Cardiology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology;  
The present study aims to explore the expression of angiotensinⅡ type 1receptor agonistic autoantibody(AT1-AA)in hyperthyroidism rats associated with cardiac hypertrophy and its relationship with miRNA-350.Thirty-six SD rats were randomly divided into control group and hyperthyroidism group.Using pathology,ELISA,RT-PCR and Western blotting methods,indexes of cardiac hypertrophy,serum AT1-AA,miRNA-350,mRNA and protein of angiotensin Ⅱ type 1receptor(AT1R)were compared between hyperthyroidism rats and the control rats.Hyperthyroidism rats were subdivided into AT1-AA positive group and negative group based on the levels of AT1-AA.The expression of miRNA-350 and AT1R were compared between these two groups.The results showed that,compared with the normal controls,rats with hyperthoidism group showed higher cardiac indexes,higher rate of AT1-AA positivity as well as higher A value.Besides,expression of miRNA-350 and AT1R were markedly higher in the diseased rats,compared with the normal rats.Furthermore,when AT1-AA positive group was compared with AT1-AA negative group,miRNA-35 and At1Rlevels were significantly elevated.These results indicate that serum AT1-AA maybe involved in the pathogenesis of cardiac hypertrophy in hyperthyroidism,and miRNA-350 could play an important role in the production of AT1-AA.
【Fund】: 武汉市科技局科研项目(2013060602010244)
【CateGory Index】: R581.1
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