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《Current Immunology》 2015-01
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Studies on the activation of murine bone marrow-derived mast cells pulsed with Brucella WobA~(-/-)S_2

WANG Xiao-man;RONG Rui-xue;WANG Jing-ru;CUI Yun;YANG Xiao-jia;LI Xuan;ZHENG Jun-wen;CAO Zhi-ran;Department of Immunology,School of Basic Medical Science,Hebei University;  
This study investigated the effects of B.suis WboA-/-S2 on the activation of bone marrow-derived mast cells(BMMC).The BMMC were pulsed by B.suis WboA-/-S2 in vitro and B.Suis S2 acted as the control.The expression of TLR4 and TLR8after infection were observed by RT-PCR at different time points.The levels of TNF-α,IL-6,IL-1and IL-12 in the serum at different time points of infection were detected by ELISA.The expression of TLR4 at 12hof pulsation with both WboA-/-S2 and S2were significantly higher than that of the normal group,besides,with the WboA-/-S2-pulsed group being significantly higher than the S2-pulsed group.Then,the expression of TLR4 in both of the groups was significantly decreased at 24hpostpulsation;the expression of TLR8 at 12hof infection with WboA-/-S2 was significantly higher than that of the normal group.However,the S2-pulsed group showed no significant difference as compared with the normal group.The expression of TLR8 in both of the groups decreased at 24hpost-pulsation.The levels of both TNF-αand IL-6in the serum of WboA-/-S2-pulsed BMMC were significantly higher than those in the S2-pulsed group at 3h,6h,12 h,24h(P0.05),but IL-1and IL-12 could not be found in the serum of BMMC in both of the groups.Our results indicate that B.suis WboA-/-S2 is more easily recognized by BMMC and is much more powerful to activate BMMC than B.suisS2,besides,it can rapidly induce BMMC to secrete large number of cytokines.
【Fund】: 河北省自然科学基金(C2011204008);; 河北大学医学学科专项资金建设项目(2012A2005);; 2013年地方高校国家级大学生创新创业训练计划项目(201310075016)
【CateGory Index】: R516.7
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