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《Current Immunology》 2015-06
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Topical application of NF-κB Decoy ODN to type Ⅱ collagen induced arthritis in rats and its effects on the synovial MMP-3、IL-17and TGF-β

HU Heng-gui;QIN Shu-guo;HUANG Feng;GUAN Shi-he;WANG Feng-chao;Department of Clinical Laboratory ,General Hospital of Wanbei Coal-Electric Group;ChinaDepartment of Clinical Laboratory,The Second Affiliated Hospital of Anhui Medical University;Department of Clinical Laboratory,The First Hospital Subsidiary of Bengbu Medical College;  
To study the treatment of topical application of NF-κB Decoy ODN targeting strategy to typeⅡcollagen induced arthritis in rats and its effects on the synovial MMP-3,IL-17 and TGF-β,TypeⅡcollagen induced arthritic rats was established.The rats were divided into control group,CIA model group and experimental group.NF-κB Decoy ODN was injected into the posterior ankle articular cavity.Forty-two days after the initial immunization,the arthritis was evaluated by arthritis index,histopathological index.The levels of IL-12 and TGF-βwere detected using ELISA method,the expressions of MMP-3、IL-17 and TGF-βin the synovium were detected by immunohistochemical method.Compared with control group,the arthritis index、the levels of IL-17 and IL-12 in the joint fluid on CIA model group were significantly elevated,IOD values of positive expression of MMP-3and IL-17 on the synovial tissue were increased significantly;After received NF-κB Decoy ODN,compared with the CIA model group,the arthritis index,the levels of IL-17 and IL-12 in the joint fluid in the experimental group were reduced significantly,the levels of TGF-βwas significantly increased,IOD values of positive expression of MMP-3and IL-17 in the synovial tissue were reduced significantly,but IOD value of positive expression of TGF-βwas significantly higher.These findings indicate that NF-κB Decoy ODN could reduce arthritis symptoms significantly,delayed the progress of joint destruction,and has a good curative effect on the model of rheumatoid arthritis.
【Fund】: 蚌埠医学院科研基金资助项目(Byky1268NF);; 安徽省科技攻关项目(12010402c196)
【CateGory Index】: R446.6
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