Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Biotechnology》 1985-01
Add to Favorite Get Latest Update

CLONING AND EXPRESSION OF GENETIC DETERMINANTS FOR K88 ANTIGEN

Hong Mengmin Zhang Jingliu Cai Ruizhu (Shanghai Institute of Plant Physiology, Academic Sinica) Fan Yingyuan Yu Ming Wu Benxie(The Institute of Animal and Veterinary Sciences, Shanghai Academy of Agricultural Sciences) Xiao Junfa (Jiading County Veterinary Station)  
Acute diarrheal disease in piglets caused by enterotcxiseric strains of E.coli is prevalent globally in animal husbandary. Antigen K88 has been shown to play an essential role in the colonization of these pathogers to the small intestine of the piglets.The plasmid pTK82 was isolated from an local enterotoxigenic E. coli strain Q3 which caused diarrheal disease in piglets. It was characterized as a≈50×10~6 daltons non-conjugative plasmid containing genetic determinants for the K88ac antigen. The electron micrographs of E. coli C600 harbo(?)ing pTK82 showed the presence of K88 pili on the bacterial cell surface. This plasmid DNA was cleaved with the HindⅢ restriction endonuclease, and the fragments were ligated to similarly cleaved DNA of pBR322. The recombinant plasmid DNA pTK63 which produced K88 antigen was isolated in E.coli C600. The pTK63 DNA was partially digested with EocRI and re-ligated to form plasmid pTK90, reduced in size but still containing K88 gene. Based on the results of immunolog analysis, the nature of pilus antigen expressed by recombinant plasmid pTK 90, was identical to K88 antigen encoded by the wild type plasmid pTK82 either in E.coli C600 or in E. coli Q3. Both E. coli C600/pTK82 and E.coli C600/pTK90 are non-enterotoxigenic strains. Preliminary tests showed that they are effective in preventing diarrheal disease in piglets.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Wu Tong, Li Yopgming, He Zhongxian (Dept. of Animal and Veterinary Seience, Guizhou Agric. Coll., China 550025);The Pathogen Isolation and Identification of Piglet Yellow Scour in Guizhou Province[J];;1991-01
2 Cui Zhizhong Jiangsu Agricultural College, Yangzhou;ADHESION TO PIGLET INTESTINAL EPITHELIAL CELLS AND ELECTRON MICROGRAPH OF SOME E. COLI STRAINS LACKING K_(88) AND K_(99) ANTIGENS[J];Acta Microbiologica Sinica;1984-03
3 Liu Xiufan, Zhou Weisong, Zhang Rukuan, Yan Ding, Bai Fenglin, Zhong Xing ( Department of Animal Husbandry and Veterinary Medicine, Jiangsu Agricultural College );MONOCLONAL ANTIBODIES SPECIFIC FOR K88 ADHESION ANTIGENS OF ENTEROTOXIGENIC E.COLI AND THEIR REACTIVITIES WITH K88 POSITIVE AND NEGATIVE STRAINS[J];Chinese Journal of Animal and Veterinary Sciences;1988-01
4 LIU Shu liang,TAO Yong,WANG Hong ning,WU Qi,HUANG Yong,LIU Ping(College of Animal Science & Technology, Sichuan Agricultural University ,Ya'an 625014, China );Serotypes of pathogenic E coli in the large scale pig farm[J];SOUTHWEST CHINA JOURNAL OF AGRICULTURAL SCIENCES;2000-S1
5 Wang Zhicai… ( Institute of Veteriaary Medicine, XAAS, Wulumuqi, 830000 );Studies on serotyping Escherichia coli strains from lambs with diarrhea[J];Chinese Journal of Veterinary Science and Technology;1992-12
6 YUAN Wan-zhe~(1,2), HE Kong-wang~2, LU Cheng-ping~3(1. College of Animal Science and Technology,Hebei Agricultural Uni Laboratory of Animal and Poultry Diseases Diagnostic,Ministry of Agriculture/Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China; 3.College of Veterinary Medicine, Nanjing Agricultural Univiversity , Nanjing 210095,China);Study on biological properties K_(88)-K_(99)-987P-F_(41) tetravalent subunit vaccine against piglet colibacillosis[J];Chinese Journal of Veterinary Science and Technology;2005-01
7 YUAN Wan-zhe~(1,2), LU Cheng-ping~(1), HE Kong-wang~(2) (1. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095,China; 2. Key Laboratory for Diagnosis of Animal and Poultry Diseases, Ministry of Agriculture/Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China);Development of reverse indirect hemagglutination test to determine enterotoxigenic Escherichia coli F_(41) adhesin contents[J];Chinese Journal of Veterinary Science and Technology;2005-02
8 CUI Yudong,HOU xilin,ZHU zhanbo,ZHAO Shulan,PIAO fanze (College of animal science and fechnology,Heilongjiang August First Land Reclamation Vniversity);Methods Comparison of Extracting Pili Antigens K99 and F41 of Enterotoxigenic E coli with Heat Treatment in Different Buffers[J];;1998-05
9 Yu Lian, WangGan, Cai Weiming;Ding Rongfa(1. Zhejiang Agricultural University ,Hangzhou 310029; 2 Shanghai Institute of Plant Physiology,Academia Sinica,Shanghai 200032; 3 Fuyang Agricultural Bureau,Fuyang 311400);Prevention of experimental neonatal colibacillosis with E.coli 987P genetic engineered vaccine.[J];Journal of Zhejiang Agricultural University;1992-04
10 Gou Wanli 1 Zhou Bijun 1 Feng Yuanzhang 2 ( 1) Department of Animal Science, Guizhou University, Guiyang 550025) ( 2) Zhongkai Agrotechnical College, Guangzhou 510225);DEVELOPMENT OF PATHOGENIC STUDIES OF COLIBACILLOSIS OF PIGLETS[J];JOURNAL OF ZHANGKAI AGUOTECHAICAL COLLEGE;1998-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved