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《Chinese Journal of Biotechnology》 1985-01
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THE TRANSFER OF LpDH ACTIVITY AS MARKER IN SOMATIC HYBRID PLANT BETWEEN TOBACCO TUMOUR B6S3 AND NORMAL TOBACCO XANTHI

Li Xianghui Schieder,O. Huang Meijuan Li Wenbin (Institute of Genetics, Academia Sinica, Beijing)(Max-Planck-Institute fur Zuchtungsforshung Koln, F. R. G.)  
The protoplasts from tobacco tumor B6S3 containing activity of LpDH but no regeneration have been fused with mesophyll protoplasts from normal Tobacco xanthi with traits contrary to the tobacco tumor. The regenerated calli from fused protoplasts have regenerated into whole plants. The results of electrophoresis for LpDH activity assayed from 65 plants suggested that 76% of the plants contained octopine spot with different degree. In some plants, the LpDH activity is reduced or totally lost during the course of development. Morphology of leave has partial traits from both parents, suggesting that the genetic marker-LpDH activity of the tobacco tumor was possiblly transferred into normal cell of Tobacco xanthi and formed somatic hybrid.
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【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 Wang Fu-de; Wan Xin-shan; Xia Zhen-ao;Hsia, C.A. Shanghai Institute of Plant Physiology, Academia Sinica;Fusion of Protoplasts from Tumour B6S3 Cells with Mesophyll of Tobacco and the Characteristics of Regenerated Plants and Their Progenies[J];Plant Physiology Communications;1986-02
2 Liu Bao, Xing Miao, Zhang Zhong heng, He Meng yuan and Hao Shui (Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024) Gu De feng, Zhao Ran and Wu Xin kang (Labaratory of Biotechnology, Jilin Agricultural University, Ch;INTERGENERIC SOMATIC HYBRID PLANTS OF NICOTIANATABACUM L. AND LYCIUM BARBARUM L. BY PROTOPLAST ELECTROFUSION[J];Acta Botanica Sinica;1995-04
China Proceedings of conference Full-text Database 1 Hits
1 Zhang Xinguo, Chen jinfen, and Liu Peiying (Deptment of Horticulture., Southwest Agriculture. University., Chongqing 400716);Culture and Fusion of Cucumber Protoplasts[A];[C];1998
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Li Wen-pin Sun Yung-ju Li Hsiang-hui(Institute of Genetics, Academia Sinica);REGENERATION OF PLANTLETS FROM ISOLATED PROTOPLASTS IN PETUNIA HYBRIDA L.[J];Acta Genetica Sinica;1978-01
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