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《Chinese Journal of Biotechnology》 1988-01
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EXPRESSION OF E COLI GALK AND GPT GENES IN OOCYTES OF XENOPUS LAEVIS

Huang He Yu Jiankang Lu Deyu (Institute of Developmental Biology,Academia Sininca,Beijing)  
Recombinant plasmids,pSVK100,pPB12,pPB22 and pPB22H which con-tain E.coli galk and gpt genes,were introduced into the germinal vesicle ofXenopus laevis oocytes by microinjection.After incubation at 19℃ for 40h,starch gel electrophoresis was used for assaying the enzyme activity.It wasshown that the expression of E.coli galk and gpt genes in Xenopus oocytesdepended on the presence of SV40 early promoter.In contrast,E.coli galkgene did not show any activity in Xenopus oocytes when only mouse β-globinpromoter was present.Our results also showed that HaSV enhancer stimulatedthe expression of E.coli gpt gene in occytes of Xenopus Laevis.
【Fund】: 中国科学院科学基金资助课题 并得到美国洛氏基金 RF84031资助
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