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《Chinese Journal of Biotechnology》 1993-01
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Cloning of the Shiga Toxin Gene and High Level Expression of B Subunit in Escherichia coli K-12

Su Guofu Li Fengsheng Huang Peitang Rui Xiangliang Huang Cuifen (Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100850)  
In this study we have cloned the gene encoding the Shiga toxin(Stx) from the chromosomal DNA of S.dysenteriae type 1 (W30864) . The Stx gene was located in a 4.5 kb EcoR1 fregment. A series of biological experiments showed that the hybrid plasmid pMGC001 containing Stx gepe could produce Sbiga toxin, and the amount of the Stx produced by pMGC001 was 16 fold more than that produced by its parent strain. The cloned strain had cytotoxic add enterotoxic activities, also neurotoxin. The gene for Stx-B subunit was subcloned into plasmid pJLAS03 from plasmid pMGC001. We obtained the high level expression of Stx-B in E. coli K-12. The Stx-B has been purified in large quantities. The polyclonal and monoclonal antibodies against Stx-B were raised. Western blot sbowed that they can react specifically to the B subunit.
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【References】
Chinese Journal Full-text Database 2 Hits
1 YU Hua-ying, WANG Jing-lin, GAO Feng, KANG Lin, ZHAO Jin-hong, WU Dong-lin ( Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China; Department of Animal Sciences, Quartermaster University of PLA, Changchun 130062, Jilin, China; Department of Detection, No. 208 Hospital of PLA, Changchun 130062, Jilin, China);Fusion Expression of Shiga Toxin B Subunit of Shigella dysenteriae Type I in E, coli and its Generation of Specific Antibody[J];Life Science Research;2004-01
2 Su Guofu (Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071);Advance in shigellosis vaccine[J];Letters in Biotechnology;1997-02
【Co-references】
Chinese Journal Full-text Database 3 Hits
1 Rui Xianliang Xu Yongqiang Wang Hong Su Guofu Huang Cuifen (Institute of Biotechnology, Beijing 100071);Construction of Stable and Non-resistant Bivalent Vaccine Candidate Strain Against Shigella flexneri 2a and Shigella sonnei[J];Chinese Journal of Biotechnology;1996-02
2 LU RONG-ZHU; CHEN CHUAN-FEN; LIN HUI-FEN;HUANG LEI-MING AND JIN XI-PENG.(Department of Preventive Medicine, Zhenjiang Medical College, 3 YizhengRoad, Zhedeng, 212001 China)(Department of Occupational Health,School of Public Health, Shanghai Medical Univer;Preliminary Validation of Tumor Cell Attachment Inhibition Assay for Developmental Toxicants With Mouse S180 Cells[J];生物医学与环境科学(英文版);1999-04
3 YU Huaying,WANG Jinglin ,GAO Feng ,RANG Lin ,WU Donglin ,ZHAO Jinhong (1. Institude of Microbiology and Epidemiology,Academy of Military Medical Sciences,Beijing,100071 ; 2.University of Land Reclamation and Cultivation in Talimu,Alaer,Xinjiang,843300; 3. Animal Science Department,University of military supplies of P.L. Army in Changchun.Jilin, 130062; 4.Examination Department, No208 Hospital of P. L. Army in Changchun,Jilin,130062);Cloning and Sequence Analysis of Shiga Toxin A, B Gene Subunits from Shigella dysenteriae Type I[J];Journal of Microbiology;2003-04
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1 MEI Ming-Zhu1 YAN Ya-Xian 2 LU Cheng-Ping 1,2(College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095)1(School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 201100)2;Prokaryotic Expression of Vero Toxin Ⅱ B Subunit from Escherichia coli O157 and Preparation of Monoclonal Antibodies[J];Microbiology;2007-03
2 Zhong Qingping He Yanping Zhong Shiqing Ge Cuicui (College of Food Science, South China Agricultural University, Guangzhou 510642);Studies on Yolk Immunogloblin against Shigella Dysenteriae[J];Journal of Chinese Institute of Food Science and Technology;2006-01
China Proceedings of conference Full-text Database 1 Hits
1 MEI Ming-Zhu~1 YAN Ya-Xian~(2**) LU Cheng-Ping~(1,2) (1 College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095; 2 School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240);Prokaryotic Expression of Vero Toxin Ⅱ B Subunit from E.coli O157 And Preparation of Monoclonal Antibodies[A];[C];2008
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