Expression of Marek's Disease Herpesvirus A Antigen Gene in Prokaryotic System
Yang Baohua Ming Yongji~* Wang Qisong~* Chen Puyan Cai Baoxiang (Nanjing Agricultural University, Nanjing 210014) (?)udan University, Shanghai 200433)~*
Sepereted 1.4kb MDV A antigen gene was ligased to pKK223-3 plastaid and transformed to E.coli JM105 strain. As the result, three expression clones have been obtained by situ-bybridization and restriction enzyme digestion. Growing cells to midlog phase at 37℃ adding IPTG and continuing growth, the MDV A antigen was expressed and produced by the three strains through Dot-ELISA screening and detecting. Furthermore, the molecular weight of expressed products is 56kDa, with SDS-PAGE and Western-blotting experiments, responding to previous reports.