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《Chinese Journal of Biotechnology》 2001-01
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Cloning of Neutral Phytase Gene nphy from Bacillus subtilis and its Expression in Escherichia coli

YAO Bin 1* YUAN Tie\|Zheng 1 WANG Yuan\|Huo 1 CAO Shi\|Shu 1 WANG Ya\|Ru 1 SHI Xiu\|Yun 1 FAN Yun\|Liu 2 1(Feed Research Institute,Chinese Academy of Agricultrual Sciences,Beijing 100081,China) 2(Biotechnology Research Cente  
The gene encoding the neutral phytase nphy was cloned from Bacillus subtilis by polymerase chain reaction(PCR). Nucleotide sequence analysis of nphy revealed the presence of an open reading frame of 1152 bp coding for 383 aa. The start codon was followed by a sequence coding for a putative signal peptide of 26 aa in length. The nphy without original signal peptide encoding sequence was cloned into E.coli expression plasmid pTYB40. The result of SDS PAGE of the phytase expressed in E.coli showed that the nphy had been overexpressed. The expressed phytase was over 40% of the total soluble protein of E.coli ,and has normal bioactivity.
【Fund】: 国家高技术发展与计划资助项目!(10 1 0 3 0 5 0 1)&&
【CateGory Index】: Q78
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【References】
Chinese Journal Full-text Database 10 Hits
1 HUANG Guang-xiang1,2,ZHOU Zhi-gang2,HE Su-xu2,SHAO Na2,SHI Peng-jun2,LIU Yu-chun2,YAO Bin2(1.Huazhong Agricultural University,Wuhan 430074;2.Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China);Studies on the Intestinal Enzyme-Producing Microflora in Typical Marine Finfish Scarus ovifrons Temminck et Schlegel,1846 at Sublayer of the South China Sea[J];Journal of Agricultural Science and Technology;2007-05
2 Gao Ming Zhao Guoxian Li Shuang'an (Hebei Agricultural University Hebei 071001,China);Application of phytase to the feedstuff of Fishes[J];Hebei Fzsheries;2004-06
3 FENG Zhihua, ZHAO Guoxian(Hobei Agricultural University Hebei, 071001, China);Application of Plant Acid Ferment to the Non-Ruminant Animals[J];Ecology of Domestic Animal;2003-02
4 Yang Fan1,2 Guo Anping1, 2 Guo liqiong1, 3 He Lika1, 2 (1 State Key Laboratory of Tropical Crops Biotechnology, CATAS, Haikou, 571101; 2 Institute of Tropical Bioscience and Biotechnoloyg, CATAS, Haikou, 571101; 3 Department of Biotechnology, College of Food Science, South China Agriculture University, Guangzhou, 510640);Screening Phytase-Producing Strains From Medical Fungi and Cloning Phytase Gene Fragment[J];Journal of South China University of Tropical Agriculture;2005-01
5 GUAN Wei(Liaoning Province Agricultural Technical School,Shenyang 110161,China);Phytase’s Currently Research and Application Prospect[J];Inner Mongolia Agricultural Science and Technology;2009-02
6 FAN Shou-cheng ZHANG Yun-ru WANG Xiao-you ZENG Xiu WANG Jin-yong FAN Shou-jun(Chongqing Pig Breeding Science Research Institute Key Laboratory of Chongqing City Chongqing 402460, China) (Chongqing Institute of Technology Bioengineering Institute Chongqing 400050, China);Construction of A New Phytase Gene Expression Vector and Its Over Expression in E. coli[J];Progress In Biotechnology;2005-01
7 ZOU Li-kou1,WANG Hong-ning1,WU Qi2,ZHAO Hai-xia1(1.Lab of Animail Prevention Medicine and Bioengineering of Sichuan Agriculture University,Ya’an 625014;2.College of Life Sicence,Sichuan Univeristy,Chengdu 625014;3.Dujiangyan Campus of Sichuan Agriculture Umiversity,Chengdu 611830,P.R.China);Construction,Identification and Expression of Recombinat Fusion Expression Vector of phyC Phytase[J];Biotechnology;2004-03
8 LIU Shengfeng,PENG Yuanyi(College of Animal Science & Technology, Southwest Agricultural University, Chongqing 400716,China);Advances in Gene Engineering of Phytase[J];Life Science Research;2003-S1
9 WU Qi WANG Hong-ning LIU Shi-gui College of Life Science, Sichuan Agricultural University Ya'an 625014, China College of Animal Sci. & Tech., Sichuan Agricultural University Ya'an 625014, China College of Life Science, Sichuan University Chengdu 610064, China;Advances on Molecular Biology of Phytase from Bacillus[J];Progress In Biotechnology;2005-S1
10 WANG Hong-ning 1,XIE Jing 2,WU Qi 2,LIU Shi-gui 2,CHEN Hui 1 (1 College of Ani Sci & Tech ,Sichuan Agricultural University Ya'an 625014;2 College of Life Science,Sichuan University,Chengdu 610064,China);Study on the Aspergillus niger N25 Strain Producing Phytase and its Phytase Reaction Conditions[J];Biotechnology;2003-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Li li,ZHANG Ling hua,SHI Jian fei,WANG Yun ji (Dept. of Food Sci. and Biotechnol., Dalian Inst. of Light Ind., Dalian 116001,China);Isolation and molecular biological analyses of genomic DNAs from fungi using benzyl chloride[J];JOURNAL OF DALIAN INSTITUTE OF LIGHT INOUSTRY;2000-01
2 WANG Jing,ZHANG Linghua,WANG Yunji(Coll. of Bio. & Food Technol., Dalian Inst. of Light Ind., Dalian 116034, China);Expression of the Inulinase gene from Kluyveromyces marxianus in Pichia pastoris[J];Journal of Dalian Institute of Light Industry;2003-02
3 YUAN Wen jie,ZHANG Ling hua,WANG Chen xiang,WANG Yun ji (Dept. of Food Sci.and Biotechnol., Dalian Inst. of Light Ind. Dalian 116001,China);Construction of phytase A gene engineering vector in plant[J];Journal of Dalian Institute of Light Inoustry;2000-04
4 XU Qi-you,JIN Ming-yu,SUN Yu-hong,WANG Li-juan,SHAN An-shan(Animal Nutrition Institute,Northeast Agricultural University, Harbin 150030 ,China);EFFECTS OF SUPPLEMENTAL MICROBIAL PHYTASEON PERFORMANCE AND UTILIZATION OFDIETARY CALCIUM,PHOSPHORUS IN BROILERS FED CORN-SOYBEAN DIETS[J];ACTA ZOONUTRIMENTA SINICA;2000-01
5 Qiu Xiaoying, Zhu Honghui, Lu Qiuyan, Qiu Yuansheng (Guangdong Institute of Microbiology, Guangzhou 510070);Study on Stability of Plasmid pMC73A Harbored in host Bacteria[J];HIGH TECHNOLOGY LETTERS;1998-06
6 XU Qin-kun et al(College of Yingdong Bioengineering of Shaoguan University,Shaoguan,Guangdong 512005);Research on the Phytase and its Application[J];Journal of Anhui Agricultural Sciences;2007-02
7 Liu Hongxing Wu Jianliang Xu Song Lu Yuli Xu Yaoxing Xu Shaochun(Zhejiang Academy of Agricultural Sciences,Hangzhou,310021);THE EFFECT OF SUPPLEMENTAL PHYTASE TO DIETS WITH VARYING LEVELS OF PHOSPHORUS ON GROWTH PERFORMANCE AND PHOSPHORUSUTILIZATION FOR BROILERS[J];ACTA ZOONUTRIMENTA SINICA;1998-01
8 ZHAO Xiang, LI Zhi, LU Shen feng, HUO Ke ke, LI Yu yang (State Key Laboratory of Genetic Engineering);Synonymous Codon Usage in Yarrowia lipolytica[J];JOURNAL OF FUDAN UNIVERSITY;1999-05
9 Wang Shihua, lu maozhou,et al;Application condition and research advance of Phytase[J];Guangzhou Food Science and Technology;2002-01
10 JIANG Shou-qun 1, WU Tian-xing 2(1.Laboratory Animal Centre of Zhejiang Province,Hangzhou 310013 ,China; 2.Zhejiang University,Hangzhou 310029 ,China);ADVANCES ON PHYTASE RESEARCH:A REVIEW[J];ACTA ZOONUTRIMENTA SINICA;1999-03
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 WEN Hai-yan1,QIN Xiao-bing 1,WEN Jian-xin1,SHAN Hu1,NIU Jin-guo2,NIE Yu-xia3(1.College of Animal Science and Technology,Qingdao Agricultural University,Qingdao,Shandong,266109,China;2.Institute of husbandry and veterinary,Agricultural Academy of Shanxi,Taiyuan,Shanxi,030032,China;3.Sericulture Research Institute of Shandong Province,Yantai,Shandong,265147,China);Cloning and Sequence Analysis of pgsA Gene of Bacillus subtilis[J];Progress in Veterinary Medicine;2008-04
2 HU Yong et al(Department of Bioengineering,Chongqing Institute of Technology,Chongqing 400050);Isolation of a Phytase-producing Aspergillus.sp Strain and Optimization of its Fermentation Conditions[J];Journal of Anhui Agricultural Sciences;2005-11
3 PENG Yi-qiang~1,PENG Kun~1,HE Yan-cai~1,FANG Bai-san~1,ZHANG Wen-zen~2(1.Department of Bioengineering,Huaqiao University,Quanzhou 362021,Fujian,China;2.College of Information and Engineering,Huaqiao University,Quanzhou 362021,Fujian,China);Laser Mutation and Purification of Phytase[J];Fine Chemicals;2005-08
4 Kang Lixin Ma Lixin Zhang Guimin(College of Life Science,Hubei University,Wuhan 430062,China);Inducing Conditions of Pichia pastoris GS115/phyA Producing Phytase[J];Journal of Huazhong Agricultural;2005-05
5 LEI Zheng-yu,HE Li-hua,LI Zhang-wu(Hubei Vocational College of Ecological Engineering,Wuhan 430200,China);On Exogenous Gene Expression in Prokaryotes and Eukaryotes[J];Journal of Hubei Vocational College of Ecological Engineering;2008-03
6 Gao Ming Zhao Guoxian Li Shuang'an (Hebei Agricultural University Hebei 071001,China);Application of phytase to the feedstuff of Fishes[J];Hebei Fzsheries;2004-06
7 LIU Fang~1,LIU Zhen~1,CHENG Jia~1,FU Gui-hong~2,WANG Huan~1,HUANG Ying~1,ZHANG Jian-she~(1*)(1.Department of Bioengineering and Environment Science,Changsha University,Changsha 410003,Hunan,China;2.College of Animal Science,Hunan Agriculture University,Changsha 410128,Hunan,China);Construction of Bacterial Phytase Gene in pEGFP-N_3 Vectors and Its Expression in COS7 Cells[J];Acta Laser Biology Sinica;2006-06
8 ZHANG Guo-hua1,2,LUO Hui-ying2,CHEN Jin-chun1,YAO Bin2(1.College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029;2.Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China);Pilot Studies on Enzyme-Producing Microflora in Frozen Soil at the Root of Saussureae involucratae Kar.et Kir.et Maxim from Tianshan,Xinjiang Autonomous Region[J];Journal of Agricultural Science and Technology;2008-02
9 ZHOU Zhi-gang1,2,SHI Peng-Jun1,YAO Bin1,HE Su-xu1,SU Yong-quan2(1.Feed Research Institute of Chinese Academy of Agricultural Sciences,Beijing 100081,China;2.Marine Biology Institute of Shantou University,Shantou 515063,China;3.Marine & Environment College of Xiamen University,Xiamen 361005,China);Comparison of the PCR-DGGE fingerprints in the gastrointestinal predominant bacterial community in Lutjanus sebae[J];Marine Sciences;2008-11
10 GUAN Wei(Liaoning Province Agricultural Technical School,Shenyang 110161,China);Phytase’s Currently Research and Application Prospect[J];Inner Mongolia Agricultural Science and Technology;2009-02
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