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《Chinese Journal of Biotechnology》 2001-01
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Immunologic Properties of coli Surface Antigen 6(CS6) of Enterotoxigenic Escherichia coli and Cholera Toxin Subunit B Expressed in Shigella flexneri 2a Strain T32

YING Tian\|Yi 1,2 \ HAN Zhao\|Zhong 1* \ FENG Er\|Ling\ WANG Heng\|Liang ZHANG Zhao\|Shan 1\ SU Guo\|Fu 1\ HUANG Cui\|Fen 1 1(Institute of biotechnology,Beijing 100071,China) 2(Institute of Pharmaceutical Chemistry,Beijing 100205  
A host\|plasmid balancing system composed with a Δ asd mutant (FaD) of an avirulent strain (T32) of Shigella flexneri 2a and plasmid harboring asd gene was used to express enterotoxigenic E.coli surface antigen 6(CS6) and V.cholerae toxin subunit B (CTB).The results of Western blotting and ELISA showed that all of recombinant plasmids (pYX201,pYX202 and pYX203) could be maintained stably and expressed CS6 and CTB respectively in T32 without any antibiotic selection.All the recombinant bacterial strains could elicit the corresponding antibodies in rabbits.The antibodies against CTB elicited by both FaD/pYX201 and FaD/pYX203 showed to be high level,and had long prolongation time,in otherwise,the antibodies against CS6 showed to be low level,indicating that higher expression level of foreign antigen may be benefit for construction of genetic multivalent vaccine.
【Fund】: 军队重点基金!(96 2 5 2 2 5 )&&
【CateGory Index】: Q939.91
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【References】
Chinese Journal Full-text Database 6 Hits
1 JIANG Xuan-peng;ZHANG Huan-rong;College of Life Science and Technology,Southwest University for Nationalities;Key Laboratory of Sichuan Institutes of Higher Learning;;Cloning and Prokaryotic Expression of Enterotoxigenic E.coli K99 Fimbria Gene[J];动物医学进展;2014-05
2 WANG Rong-rong,MA Yong-ping(Research Center of Medical Molecules and Tumors,Department of Biochemistry and Molecular Biology,College of Basic Medicine,Chongqing Medical University,Chongqing 400016,China);Progress in Development of Recombinant Human Enterotoxigenic Escherichia coli Vaccine[J];中国生物制品学杂志;2010-05
3 QIU Su-jun~3,LV Chun-hua~2,YANG Dong-mei~1 (l.Turpan Prefecture Center of Animal Disease Control and Diagnosis in Xinjiang,Turpan 838000,China; 2.Altai Prefecture Center of Animal Disease Control and Diagnosis in Xinjiang,Altai 836500,China);The Research on Enterotoxigenic Escherichia Coil[J];草食家畜;2009-03
4 YANG Dong-mei,CHEN Chuang-fu,WANG Peng-yan,REN Yan (Laboratory of Xinjiang Endemic and Ethnic Diseases,Shihezi University,Shihezi,Xinjiang 832000,China);Construction and Identification of a Reconbinant Live Attenuated Salmonella typhimurium Vaccina Strain Carrying K88ac-STII Fusion Gene of Enterotoxigenic Escherichia[J];石河子大学学报(自然科学版);2007-03
5 LI He~(1),MENG Qing-wen~2,L Ping~1,MA Bao-hua~1,YAN Zhi-qiang~1(1.Nanhai Entry-exit Inspection and Quarantine Bureau,Foshan,Guangdong,528200,China;2.Harbin Veterinary Research Institute,CAAS,Harbin,Heilongjiang,150001,China);Progress on Bacterial Vector Vaccine[J];动物医学进展;2006-S1
6 Wang Tao 1 Chen Jianping 1,2Δ Zhang Lei 1 Wang Yufang 3 Ma Ying 1 1(Department of Parasitology, West China Medical Center, Sichuan University, Chengdu 610041,China) 2(Department of Morphology, West China Medical Center, Sichuan University, Chengdu 610041,China) 3(Department of Pathology, West China Medical Center, Sichuan University, Chengdu 610041,China);Cloning and Expression of Immunoadjuvant Molecule-CTB Gene[J];生物医学工程学杂志;2004-02
【Citations】
Chinese Journal Full-text Database 2 Hits
1 Qian Feng;Rui Xianliang;Su Guofu;Huang Cuifen(Institute of Biotechnology,Academy of Military Medical Science Beijing 100850);Expression of S.sonnei form Ⅰ Antigen Gene and V.cholerae Toxin B Subunit Gene in S. flexneri 2a Strain and Investigation of Their Immunoprotective Response in Mice[J];遗传学报;1996-04
2 Yang Xiao Zhang Zhaoshan Liu Chunjie Zhang Beining Chen Tianmi Huang Cuifen(Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100850);CLONING OF A CLUSTER OF GENES ENCODING COLI-SURFACE ANTIGEN 6(CS6) OF HUMAN ENTEROTOXIGENIC E.coli[J];微生物学报;1995-05
【Co-citations】
Chinese Journal Full-text Database 6 Hits
1 LUO Gang, YANG Xiao, LI Shu-Qin, ZhANG Zhao-Shan * (Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China);High expression of CS6 colonization factor of enterotoxigenic Escherichia coli (ETEC) in Escherichia coli[J];军事医学科学院院刊;2004-03
2 LUO Gang 2) , LI Shu Qin 1) , WANG Ling Chun 1) , ZHAO Ying 2) , ZHENG Ji Ping 1) , DUAN Hai Qing 1) , ZHANG Zhao Shan 1)** ( 1) Beijing Institute of Biotechnology, Beijing 100071, China; 2) College of Life Science, JiLin University, Changchun 130023,China);Expressing CS6 of Enterotoxigenic Escherichia coli by Attenuated Shigella flexneri 2a[J];生物化学与生物物理进展;2004-02
3 YING Tian_Yi, HAN Zhao_Zhong, WANG Heng_Liang, FENG Er_Ling,ZHANG Zhao_Shan, SU Guo_Fu (Institute of Biotechnology,Academy of Military Medical Sciences,Beijing 100071,China);Coexpression of CS6 of ETEC and CTB in a genetic avirulent Salmonella typhi strain and the immunologic properties[J];军事医学科学院院刊;2003-01
4 HUANG Wei,ZHONG Hui,CAO Cheng(Institute of Biotechnology,Bejing100850,China);The construction and application of chromsome-plsmid balanced-lethal system[J];生物技术通讯;2002-05
5 YING Tian\|Yi 1,2 \ HAN Zhao\|Zhong 1* \ FENG Er\|Ling\ WANG Heng\|Liang ZHANG Zhao\|Shan 1\ SU Guo\|Fu 1\ HUANG Cui\|Fen 1 1(Institute of biotechnology,Beijing 100071,China) 2(Institute of Pharmaceutical Chemistry,Beijing 100205;Immunologic Properties of coli Surface Antigen 6(CS6) of Enterotoxigenic Escherichia coli and Cholera Toxin Subunit B Expressed in Shigella flexneri 2a Strain T32[J];生物工程学报;2001-01
6 Teng Jiabo;Rui Xianliang; Su Guofu; Zhang Yi(Changchun Instiute of Biological Products; 2 Dept. of Toxicity, NBUMS);Establishment of the stable delivery foreign antigens with double aro attentuated salmonlla typhi as carrier[J];白求恩医科大学学报;1999-02
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 XU Gang et al(Chengdu Vocational College of Agricultural Science and Technology,Wenjiang,Sichuan 611130);Gene Cloning and Prokaryotic Expression of Adhesin in Enterotoxigenic Escherichia coil from Swine[J];安徽农业科学;2012-24
2 Wen Lijuan1,Wang Guihua1,Hou Xilin2,Yu Liyun1,2,Wang Meng1(1.College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319;2.College of Animal Science and Technology,Heilongjiang Bayi Agricultural University);The Surface Display of ETEC K99,K88 Protein in Lactobacillus casei[J];黑龙江八一农垦大学学报;2011-03
3 HUANG Xue-ping,et al (Department of Biochemistry and Molecular Biology,College of Basic Medicine, Chongqing Medical University);Cloning and expression of escherichia coli colonization factor antigen CFA/Ⅰ in escherichia coli DH5α[J];重庆医科大学学报;2008-07
4 YU Di1,FU Haibing1,DING Lin1,SHI Dongfang1,SHAN Anshan2(1.College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China; 2.College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,China);Clone and expression of pili subunit gene from adhesion F5 of the enterotoxigenic Escherichia coli(ETEC)[J];东北农业大学学报;2008-06
5 LUO Yao-ling,WANG Yong,HUANG Xue-ping,RAN Dan-xia,MA Yong-ping,SONG Fang-zhou(Department of Biochemistry and Molecular Biology,College of Basic Medical Science,Chongqing Medical University,Chongqing 400016,China);Expression of B-subunit of the Escherichia coli heat-labile enterotoxin in Bifidobacterium infantis[J];中国微生态学杂志;2008-02
6 WEN Xiao-bo,CUI Yu-dong,RAN Xun-hua,ZHU Zhan-bo, PIAO Fan-ze,PAN Xing-guang(Heilongjiang August First Land Agricultural University, Daqing 163319,China);Clone and expression of pili subunit gene from the pathogenic factor adhesion F41 and K99 of the Enterotoxigenic Escherichia coli(ETEC)[J];中国人兽共患病学报;2007-08
7 ZHANG Ying-dan,XU Wei-ming,LI Jian-feng(Institute of Medical Biology,Chinese Academy of Medical Sciences,Kunming,Yunnan,650118,China);Prgoress on Escherichia coli Heat Labile Enterotoxin[J];动物医学进展;2007-02
8 ZENG Nian-hua,WANG Zhi-bin,JIANG Pu-lin,ZHANG Zhao-shan,CHEN Huan-yong(Center for Disease Control and Prevention,Guangzhou Command,Guangzhou 510507,China);IMMUNOGENICITY OF ENTEROTOXIGENIC ESCHERICHIA COLI GENETIC VACCINE IN MOUSE[J];解放军预防医学杂志;2006-04
9 Ji-Ping Zheng, Zhao-Shan Zhang, Shu-Qin Li, Xiang-Xin Liu, Sheng-Ling Yuan, Peng Wang, De-Wen Zhan, Ling-Chun Wang, Cui-Fen Huang, Beijing Institute of Biotechnology, Beijing 100071, China;Construction of a novel Shigella live-vector strain co-expressing CS3 and LTB/STm of enterotoxigenic E.coli[J];World Journal of Gastroenterology;2005-22
10 YANG Xiao-feng~1,CHEN Hong-wei~1,ZHENG Jin~1,SI Lu-sheng~1,DONG Xiao-ping~2,WANG Yi-li~1 (1.The Key Laboratory of Ministry of Education for Biomedical Information Engineering,Institution for Cancer Research of Life Science and Technology, Xian Jiaotong University,Xian 710061,China; 2.National Institute for Viral Disease Control and Prevention,China CDC,Beijing 100052,China);Construction,Characterization and Animal Testing of Attenuated Shigella Flexneri 5a sh42 Recombinant for Human Papillomavirus Type 16 L1 Protein[J];病毒学报;2005-03
【Secondary References】
Chinese Journal Full-text Database 7 Hits
1 XIE Jin-wen;LI Shu-guang;XIAO Yue-qiang;WANG Wen-xiu;TANG Na;DONG Lin;LI Feng;SHEN Zhi-qiang;Binzhou Institute of Animal Science & Veterinary Medicine,Shandong Research & Development Center of Veterinary Propolis Vaccines;Shandong Lvdu Biotechnology Co.Ltd.;;Preliminary Study on High Density Fermentation Thchnology of Gene Engineering Bacteria K88-K99 Fusion Protein[J];动物医学进展;2015-10
2 BAO Shao-wen;ZHUANG Pei-pei;SHI Juan;YANG Jia-li;ZENG Jin;LI Wu;LIU Xiao-ming;DENG Guang-cun;Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western,Ningxia University;College of Life Science, Ningxia University;;Immunoprotection of mice with recombinant adenovirus expressing STa-K99 fusion protein of enterotoxigenic Escherichia coli[J];中国预防兽医学报;2014-12
3 DENG Guang-cun;BAO Shao-wen;YANG Ji-hui;ZENG Jin;LI Wu;LIU Xiao-ming;Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western,Ningxia University;College of Life Science,Ningxia University;;Construction and Expression of Recombinant Adenoviral Vector with STa-K99 Fusion Protein of Enterotoxigenic Escherichia coli[J];动物医学进展;2013-10
4 TANG Da,BI Hai-lin,TANG Jian-hua(Department of Veterinary Medicine,Southwest University,Rongchang,Chongqing,China,402460);Progress in genetic engineering vaccine[J];畜禽业;2012-11
5 JIANG Ke-an,HE Zhi-liang,SONG Fang-zhou,MA Yong-ping(Department of Biochemistry and Molecular Biology,Molecular Medicine&Cancer Research Center,Chongqing Medical University,Chongqing 400016,China);Prokaryotic expressions of K88 fimbrial protein of enterotoxigenic E. coli and E. coli heat-labile enterotoxin b subunit and their immune effects by intranasal route[J];中国生物制品学杂志;2012-09
6 HE Zhi-liang,JIANG Ke-an,SONG Fang-zhou,MA Yong-ping(Department of Biochemistry and Molecular Biology,Molecular Medicine & Cancer Research Center,Chongqing Medical University,Chongqing 400016,China);Prokaryotic expression and immunogenicity of FasA subunit of 987P fimbrial protein of enterptpxogemoc E. coli[J];中国生物制品学杂志;2012-09
7 ZHI Hai-dong1,2,WANG Yun-feng1,CHEN Hong-yan1,2(1.Chinese Academy of Agricultural Sciences,Harbin Veterinary Research Institute,State Key Laboratory of Veterinary Biotechnology,Harbin,Heilongjiang,150001,China;2.Harbin Weike Bio-technology Development Company,Harbin,Heilongjiang,150069,China);Current Status and Strategies for Research and Development of Veterinary Genetic Engineering Vaccines in China[J];动物医学进展;2011-06
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Li.Deling Su Guofu Rui Xianliang Huang Cuifen (Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100850);The Protective Effects of Shigella sonnei Form I Antigen and Vibrio cholerae CT-B Antigen[J];生物工程学报;1996-01
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