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《Chinese Journal of Biotechnology》 2001-01
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Human Angiogenin: Expression, Purification, Biological Assay

YANG Hui 1* \ ZHANG Ying\|Qi 1\ YAN Zhen 1\ HAN Wei 1\ YAO Li\|Bo 2\ SU Cheng\|Zhi 2 (1.The Fourth Military Medical University Biotechnology Center, 2.The Fourth Military Medical University Department of Biochemistry and Molecular Biology, Xi′  
Angiogenin cDNA was obtained by RT\|PCR, and cloned into the fusion expression vector pRSETB. The recombinant Angiogenin protein was fused with His6 at its N\|terminal and expressed as inclusion body. The expression level was about 10% of the total bacteria protein. After dissolved in 8mol/L urea, the recombinant protein was purified by Ni\+\{2+\}\|NTA chelating resin, according to the high affinity of His6 with Ni\+\{2+\}. The biological assay indicated that purified rhANG could induced the new blood vessel formation on CAM and degraded tRNA \%in vitro\%.
【CateGory Index】: Q78
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