Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Biotechnology》 2001-01
Add to Favorite Get Latest Update

Research on the Selecting Suspension Cell Line of Higher Productivity of Flavonol Glycoside by Hypoxia Stress as Well as the Stability in Subcultures

LIU Jia\|Jia\+ 1* \ GUO Yong\+2\ ZHENG Sui\|Ping\+2\ ZHANG Ming\|Hao\+1 1(College of Chemistry and Chemical Engineering, Central\|south University of Technology, Changsha 410082, China) 2(College of Food Engineering and Biotechnology, Sout  
Investigate the influence of culture media to growth and flavonol glycoside synthesis of calli introduced from seedling of \%Ginkgo biloba\%. 6 cell lines were selected from calli by hypoxia stress. Among these cell lines the best one TZ\|1 which growth index was 4.12 and the flavonol glycoside content was 1.25% in dried cell which was enhanced 257.1% compared with callus. The stability in subcultures was investigated: The average content of flavonol glycoside was 1.25% in dried cells and the growth index was 3.99 during 6 subcultures. Which variation coefficient was separately 0.065 and 0.048. The results show that hypoxia stress is a efficient method to select suspension cell line of higher productivity of flavonol glycoside.
【Fund】: 广东自然科学基金!(990 86 5 );; 中南工业大学基金资助项目&&
【CateGory Index】: Q943
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 10 Hits
1 LIChunbin*1,3,WANGGuanlin2,YUEYulian3,JIANGBo3,FANGHongjun2 ( 1.School of Chem. Eng., Dalian Univ. of Technol., Dalian 116012, China; 2.Life Sci. School, Liaoning Norm. Univ., Dalian 116029, China; 3.The Res. Cent. of Biological Eng., Dalian Nat. Univ., Dalian 116600, China );Research on effect of culture condition factors on synthesis of flavone glycosides in cell suspension of Ginkgo biloba L.[J];Journal of Dalian University of Technology;2003-03
2 CHEN Fa-xing~(1,2), LAI Zhong-xiong~(1,2)(1.Institue of Horticultural Biotechnology;2.Institute of Subtropical Fruit Trees, Fujian Agriculture and Forestry Uinversity, Fuzhou, Fujian 350002, China);The callus of Ginkgo biloba and its flavonol glycosides[J];;2004-04
3 He Yong 1, 2) Tian Zhihong 2) Zheng Yonglian 1)( 1)College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 2)College of Life Science, Yangtze University, Jingzhou 434025, China);Establishment and Optimization of Suspension Cell System in Creeping Dichondra (Dichondra repens Forst.)[J];Journal of Huazhong Agricultural;2005-04
4 LI Yong-xin1,2,WANG Yi-qiang3,SHU Fu-en3,LI Jun-bin3(1.Hunan Academy of Forestry,Changsha 410004,Hunan,China;2.Hunan Key Laboratory of Trees Clones Breeding Technology,Changsha 410004,Hunan,China;3.Central South University of Forestry & Technology,Changsha 410004,Hunan,China);Breeding of High-yield Flavonol Glycosides Cell Lines in Ginkgo biloba Cell Culture in Hunan[J];Nonwood Forest Research;2007-03
5 Qin Weidong Gao Mingxia Miao Jingzhi Lv Zhaoqi(Food Engineering Department of Xuzhou Institute of Technology, Xuzhou, 221008, Chian);Studies on Flavonois Production by Suspended Cultures of Immobilized Cloning Cells of Ginkgo[J];Food and Fermentation Industries;2005-07
6 GUO Chang-lu~(1,2),XU Zhong~1,DAI Zheng~2,HE Xin-hua~3,CHEN Li-geng~2,XIE Qiu-ju~1,LI Yan-zhao~1(1.School of the Ocean,Harbin Institute of Technology at Weihai,Weihai 264209,China;2.College of Agriculture and Biotechnology,Zhejiang University,Hangzhou,310029,China;3.Agriculture College of Guangxi University,Nanning 530005,China);Studies on Flavonoid and Ginkgolides Produced by Tissue Culture of Ginkgo biloba[J];Biotechnology;2006-04
7 MENG Chao,XU Hong,CHENG Ke-di~*(Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union of Medical College,Beijing 100050,China);Preliminary Study on the Ginkgolide B and Bilobalide Contents in Different Tissues and Callus Cultures of Ginkgo biloba[J];Natural Product Research and Development;2005-05
8 YANG Ying,HE Feng,JI Jia-Xing,LEI Jing,CHEN Xue-Hong,YU Long-Jiang(Institute of Resource Biology and Biotechnology,College of Life Science and Technology,Huazhong University of Science and Technology,Wuhan 430074,China);The Effect of Precursor Feeding on Flavonoids Biosynthesis in Cell Suspension Cultures of Glycyrrhiza inflata Bat.[J];Journal of Wuhan Botanical Research;2007-05
9 Zhou Peng1 Chen Wei1,2 Jiang Bo1 Chen Jie1 Chen Jian1,3 (1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122 2School of Food Science and Technology, Jiangnan University, Wuxi 214122 3Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122);Recent Advances in Food Research and Technology in China:With Emphasis on Traditional Fermented Foods and Functional Foods[J];Journal of Chinese Institute of Food Science and Technology;2009-01
10 Jie Dong,Fenghui Qi,Yaguang Zhan College of Life Sciences,Northeast Forestry University,Harbin 150040,China;Establishment of the Suspension Culture System and Optimization of Biosynthesis of Gallic Acid in Acer ginnala[J];Chinese Bulletin of Botany;2008-06
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Xie Danian;Chen Yali;Guo Zhaogui(National Training Center of Clinical Pharmacology,Hunan Medical University,Changsha,410078);A Simple and Fast Determination of Histamine in Platelet by High Performance Liquid Chromatography(HPLC)with Pre-column Derivatization with o-Phthalaldehyde[J];CHINESE JOURNAL OF CHROMATOGRAPHY;1994-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Liu Zhimin , Zhao Suoqi, Wang Ren'an, Yang Guanghua ( State Key Heavy Oil Research Laboratory, Petroleum University, Beijing 102200);Determination of the Flavonoids from Ginkgo Biloba L. Extract by Supercritical Fluid Chromatography[J];Chinese Journal of Analytical Chemistry;1999-02
2 Wang Junde , Xiong Bohui, Cong Runzi, Shen li (Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Dalian 116012);Several Problems in Analysis of Flavonoids from Ginkgo Biloba by High Performance Liquid Chromatography[J];Chinese Journal of Analytical Chemistry;1999-09
3 Shang Guan Xiao-dong (Baoji vocational and technological college,Baoji 721400) and Lang Hui-yun (Departmentof Chemistry, Northwest University, Xi′an 710069);Determination of flavonoids of Ginkgo biloba by H_2O_2-Luminol chemiluminescence inhibition with flow injection[J];Analytical Laboratory;2004-11
4 SHANGGUAN Xiao-dong, LANG Hui-yun, MA Ya-jun(Department of Chemistry, Northwest University, Xi′an 710069), and SHANGGUAN Xiao-dong (Baoji Vocational and Technological College, Baoji 721400);Indirect determination of flavonoids of ginkgo biloba by atomic absorption spectroscopy[J];Analytical Laboratory;2004-12
5 SHANGGUAN Xiao-dong, WANG Fu-sheng (Baoji vocational and technological college, Baoji 721400) and LANG Hui-yun (Department of Chemistry, Northwest University, Xi′an 710069);Determination of flavonoids of Ginkgo biloba by ClO~--Luminol flow injection with inhibited chemiluminescence[J];Analytical Laboratory;2005-03
6 Zuo Xiongjun, Li Jing (Guangzhou Institute of Chemistry,Chinese Academy of Sciences,Guangzhou 510650);HPLC Analysis of G.B.L.Flavonoids in Extracts and Residue Leaf Extracted by SF-CO_2 with and without Entrainer[J];GUANGZHOU CHEMISTRY;1999-03
7 Zhang Chunxiu, Hu Xiaoling, Yue Hong(Department of Chemical Engineering, Northwestern Polytechnological University, Xi'an, 710072);Progress of Extraction and Analytical Methods of Flavones in Ginkgo Biloba Leave[J];Chemical Industry and Engineering Progress;1999-06
8 Wang Aimin (Analysis and Testing Center of Guizhou Normal University, Guiyang 550001);ALANYSIS OF THE FLAVONOIDS FROM GINKGO BILOBA EXTRACT BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY[J];JOURNAL OF GUIZHOU NORMAL UNIVERSITY(NATURAL SCIENCE);1997-03
9 SUN Ning,ZHAO Xin-huai(Key Laboratory of Dairy Science,Ministry of Education,Northeast Agricultural University,Harbin 150030,China);Improved method for rapid determination of five biogenic amines in cheese with HPLC[J];China Dairy Industry;2008-07
10 $$$$ Yue Bisong Qiu Andong (Department of Biology);STUDIED ON THE SPATIAL PATTERN AND SAMPLING OF AGISTMUS EXSERTUS GOUZALEZ(ACARI:STIGMAEIDAE)[J];JOURNAL OF SICHUAN UNIVERSITY (NATURAL SCIENCE EDITION);1998-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Han ShuliangDepartmentofBiology,Henan Normal Universily 453002;STUDY ON THE EFFECT OF FOUR CHEMICAL CON- STITUENTS AGAINST CANCER OF SAUSSUREA INVOLU-CRATA[J];CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS;1995-02
2 Li Yong-li et al(Department of Agriculture Science,Xinyang Agriculture College,Xinyang,Henan 464000);Progress of the Research on Production of Plant Drug Secondary Metabolites by Cell Culture[J];Journal of Anhui Agricultural Sciences;2006-02
3 YUAN Xiu-yun et al (Institute of Biology Technology,Zhengzhou Teacher's College,Zhengzhou,Henan 450044);Research on Tissue Culture and Regeneration Technology of Sophora japonica[J];Journal of Anhui Agricultural Sciences;2007-35
4 YU Yong-zhu 2 , ZHENG Heng 1* , JIN Bao-chang 2 , ZHAO Yan-sheng 1 , FANG Bi-sheng 2 (1.China Pharmaceutical University,Nanjing 210009; 2.Jiangsu Shuanggou Group, Suqian 223911, China);Studies of Mutagenic Effect of Laser on Abscisic Acid-Producing Strain[J];Amino Acids & Biotic Resources;2004-03
5 Zhou jiaqi Qin kuijie Wu Dixin Wang Lianying;AN INVESTIGATION OF WILD FLOWER GERWPLASM RESOURCES IN HUO DI TANG AND OTHER DISTRICTS ALONG THE SOUTHERN SLOPES OF QIN LING[J];;1982-02
6 Chen Yaohua Dong Pengju Bai Hengqin (Department of Landscape Gardening);A Preliminary Report on the Experiment on Propagation of Ginkgo biloba in Vitro[J];Journal of Beijing Forestry University;1988-S2
7 Liu Peng; Xu Gendi; Zhang Jie.College of Life and Environment Science, Zhejiang Normal Univ., 321004,P.R.China.;Preliminary studies on callus inducement of \%Heptacodium miconioides\% Rehd.[J];Journal of Beijing Forestry University;2001-06
8 Zhang Guanghui; Chen Chunqiu; Li Jingyun; Wang Xiqiang.Plant Tissue Culture Center,Xuzhou Agricultural School,Jiangsu 221006,P.R.China.;Advances of production of secondary metabolites in vitro cluture in Ginkgo biloba.[J];Journal of Beijing Forestry University;2002-04
9 JIA Cai-feng; LI Yue; ZHANG Rong. Key Laboratory for Genetic and Breeding in Forest Trees and Ornamental Plants,Ministry of Education, College of Biological Science and Biotechnology,Beijing Forestry University,100083,P.R.China.;Organ culture of mature embryos in Pinus armandii Franch[J];Journal of Beijing Forestry University;2006-04
10 Luo Xiaofang; Tian Yanting; Li Yun; Niu Chen (Research Centre of Improved Cultivar Breeding of BFU, 100083);Study on Fast Propagation of Chinese Date ‘Jinsi Xiaozao’(Zizyphus Jujuba Mill ) with Tissue Culture[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1996-02
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 LI Yan,DONG Juan-e,JIANG Zai-min,TANG Rui (Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China);Study on dynamic accumulation of secondary metabolites in callus of Eucommia ulmoides[J];Acta Botanica Boreali-occidentalia Sinica;2004-11
2 CHEN Ying,CAO Fu-liang~*,GAN Xi-xua(College of Forest Resource and Environment,Nanjing Forestry University,Nanjing 210037,China);Protein Analysis in Ginkgo Calluses with Different Colors by Electrophoresis and Electron Microscopy[J];Acta Botanica Boreali-Occidentalia Sinica;2006-11
3 LI Yan,YANG Xiao-yan,LIN Yue-xin(Department of Biological Science and Engineering,Longyan University,Longyan,Fujian 36400,China);Effects of gingkgo leaves substituting for feed antibiotic in the broilers diet[J];;2006-04
4 YANG Xiao-yan,LIN Yue-xin,LI Yan(Department of Biological Science and Engineering,Longyan University,Longyan, Fujian 364000,China);Effect of ginkgo biloba extract on growth performance,slaughter performance and immune index in broilers[J];Journal of Fujian Agriculture and Forestry University(Natural Science Edition);2008-03
5 XU Xiao-yong,XU Yuan,KONG Fen,WANG Ru-yan(School of Horticulture and Plant Protection,Yangzhou University,Yangzhou,Jiangsu 225009,China);Callus Induction from Mature Endosperm of Ginkgo biloba L.[J];Northern Horticulture;2009-11
6 LIU Yu-min~1;LIU Ya-min~1;MA Ming~ 1,2 ;HE Bing-hui~1. 1 College of Resources and Environment,Southwest University,Chongqing,400716,P. R. China;2 College of Forestry,Northwest A & F University,Yangling District,Shaanxi Province,712100,P. R. China.;Influence of culture conditions on the growth of callus and content of total flavonoids in Acer buergerianum Miq[J];Journal of Beijing Forestry University;2010-01
7 HE Xue-li,WANG Dong-xue(College of Life Sciences, Hebei University, Baoding 071002, China);Studies on the Tissue Culture and Medical Compisition of Ginkgo biloba L.[J];Journal of Hebei University(Natural Science Edition);2005-04
8 LI Yong-xin1,2,WANG Yi-qiang3,SHU Fu-en3,LI Jun-bin3(1.Hunan Academy of Forestry,Changsha 410004,Hunan,China;2.Hunan Key Laboratory of Trees Clones Breeding Technology,Changsha 410004,Hunan,China;3.Central South University of Forestry & Technology,Changsha 410004,Hunan,China);Breeding of High-yield Flavonol Glycosides Cell Lines in Ginkgo biloba Cell Culture in Hunan[J];Nonwood Forest Research;2007-03
9 LIN Yue-xin,LI Yan,YANG Xiao-yan;Biological Functions of Extract of Ginkgo Biloba and Its Application Progress in Poultry Production[J];Journal of Longyan University;2007-06
10 GUO Xiao-bing,LOU Ying-chun,YE Dong-mei,Li Feng-ming,YNAG Hai-feng,BAI Yu-e(College of Agronomy,Inner Mongolia Agricultural University,Huhhot 010019,China);RESEARCH OF EMBRYOGENIC CELLS SUSPENSI ON CULTURE ON PICEA MONGOLICA[J];Journal of Inner Mongolia Agricultural University(Natural Science Edition);2009-02
China Proceedings of conference Full-text Database 2 Hits
1 Zhou Wei, Qiu Guanming, Li Xikun, Yan Changhao, Li Wei(Jiangsu Academy of Agricultural Sciences , Nanjing 210014 , China ;Changchun University of Science and Technology , Changchun 130022 , China ; Nanjing University of Technology , Nanjing 210009, China ; Shenyang Ligong University , Shenyang 110168 , China ;Shanghai Institute for Biological Sciences , Chinese Academy of Sciences , Shanghai 200031 , China );Rare Earth Biological Modifier in Plants and Microorganisms[A];[C];2005
2 ;Effects of the Light on Callus Growth and Secondary Metabolite[A];[C];2007
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Xiang Junjian and Cheng Huacui(Institute uf Basic Medical Sciences. Chinese Academy of Medical Sciences. Beijing);Modified Method of Fluorometric Determination of Blood Histamine[J];Acta Academiae Medicinae Sinicae;1981-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved