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《Chinese Journal of Biotechnology》 2001-05
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High-level Production of Poly (3-Hydroxybutyrate-co-3-Hydroxyvalerate) by Feb-batch Culture of Alcaligenes eutrophus

CAI Yi\|Bin\ LIU Mo\|Qing\ YI Zu\|Hua\ CHEN Qi\ WENG Wei\|Qi * (Institute of Microbiology,Chinese Academy of Sciences,Beijing 100081,China)  
Fermentation strategies for production P (3HB co 3HV) from glucose and propionic (or valeric) acid by Alcaligenes eutrophus were studied.During the culture,we controlled pH of the broth by feeding precusors of 3HV\| propionic or valeric acid after Ammonia feeding stopped.When propionic acid were used as the precusor,for 50 hours,we obtained a cell dry weight,a P(3HB co 3HV) concentration,a P(3HB co 3HV) content and a 3HV fraction of 149 9g/L,1249g/L,833% and 124mol%,respectively,with a PHA productivity of 250 gh -1 L -1 .When valeric acid were used as the precusor,for 45 hours,we obtained a cell dry weight,a P(3HB co 3HV) concentration,a P(3HB\|co\|3HV)content and a 3HV fraction of 160.2g/L,119.0g/L,74.2% and 17.7mol%,respectively,with a PHA productivity of 2.64gh -1 L -1 .Prior to this study,it hasn′t been reported to obtain such high level productivity and 3HV fraction at the same time by Alcaligenes eutrophus.
【Fund】: 国家九五攻关项目(96 C0 3 0 3 0 2 )&&
【CateGory Index】: TQ320
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【References】
Chinese Journal Full-text Database 2 Hits
1 LIU Shuang-Jiang * (Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080,China);Biosynthesis and Accumulation of Poly(3-hydroxybutyrate) in Vibrio natriegens[J];Chinese Journal of Biotechnology;2002-05
2 ZHOU Hang;YU Hui-min;SHEN Zhong-yao Department of Chemical Engineering, Tsinghua University, Beijing 100084, China;Research on PHBV fermentation with recombined E.coli VG1(pTU14)[J];Modern Chemical Industry;2003-S1
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 WANG Rong 1,ZHAO Yingyi 2,ZHANG Yunkai 1,WEI Yutuo 1,LIN Ying 2 (1 Biotechnology Research Center,Guangxi Univ.,Nanning 530005,China; 2 College of Biotechnology and Sugarcane engineering,Guangxi Univ.,Nanning 530004,China);The study of fermentation conditions of genetic engineering microorganism[J];Journal of Guangxi Agricultured and Biological Science;2002-01
2 LIU yong 1, ZHANG Chang kai 1, GAO Guang yu 2, FANG Min 1 (1.State Key Laboratory of Microbial Technology; Shandong University,Jinan 250100; 2.Department of Chemical Engineering,Yantai University,Yantai 264005);The effect of batch-feeding culture on L-isoleucine fermentation[J];INDUSTRIAL MICROBIOLOGY;2000-02
3 LIU Sen lin, ZONG Min hua, LOU Wen yong (Food and Biotechnology Dept. South China University of Technology, Guangzhou 510640);High density cultivation using feeding method by Bacillus thuringiensis[J];Industrial Microbiology;2000-04
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5 Li Shijie et al. (Department of Bioengineering, Hubei Polytechnic College,Wuhan 430068,China);Study on the Feed Batch culture technology of Bt submerged fermentation[J];Hubei Agricultural Sciences;2001-01
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7 YU Huimin 1,ZHANG Yanping 1,SHI Yue 1,YANG Shengli 2 and SHEN Zhongyao 1 ( 1 Department of Chemical Engineering,Tsinghua University,Beijing 100084,China; 2 Shanghai Research Center of Biotechnology,Academic Sinica,Shanghai 200233,China);PRODUCTION OF POLY-β-HYDROXYBUTYRATE BY FED-BATCH CULTURE OF NOVEL RECOMBINANT Escherichia coli VG1(pTU14)[J];Journal of Chemical Industry and Engineering(China);2002-07
8 GAO Hua, TANG Fang and TAN Tianwei(Department of Biochemical Engineering, Beijing University of Chemical Technology, Beijing 100029);COMPARISON AND ANALYSIS OF DIFFERENT FED - BATCH METHODS ON FERMENTATION OF ERGOSTEROL[J];Journal of Chemical Industry and Engineering(China);2002-12
9 (The State Key Laboratory of Microbial Technology of Shandong University, Jinan\ 250100);Studies on the production of PHB by fed batch culture with DO stat method employing alcaligenes eutrophus mutant B510[J];ACTA SCIENTIAE CIRCUMSTANTIAE;1999-01
10 Chen Guoqiang Wu Qiong;Biodegradable Plastics: Studies on Manufacturing Technology of Polyhydroxyalkanoates (PHA)[J];Fine and Specialty Chemicals;2001-18
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