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《Chinese Journal of Biotechnology》 2001-05
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High-level Production of Poly (3-Hydroxybutyrate-co-3-Hydroxyvalerate) by Feb-batch Culture of Alcaligenes eutrophus

CAI Yi\|Bin\ LIU Mo\|Qing\ YI Zu\|Hua\ CHEN Qi\ WENG Wei\|Qi * (Institute of Microbiology,Chinese Academy of Sciences,Beijing 100081,China)  
Fermentation strategies for production P (3HB co 3HV) from glucose and propionic (or valeric) acid by Alcaligenes eutrophus were studied.During the culture,we controlled pH of the broth by feeding precusors of 3HV\| propionic or valeric acid after Ammonia feeding stopped.When propionic acid were used as the precusor,for 50 hours,we obtained a cell dry weight,a P(3HB co 3HV) concentration,a P(3HB co 3HV) content and a 3HV fraction of 149 9g/L,1249g/L,833% and 124mol%,respectively,with a PHA productivity of 250 gh -1 L -1 .When valeric acid were used as the precusor,for 45 hours,we obtained a cell dry weight,a P(3HB co 3HV) concentration,a P(3HB\|co\|3HV)content and a 3HV fraction of 160.2g/L,119.0g/L,74.2% and 17.7mol%,respectively,with a PHA productivity of 2.64gh -1 L -1 .Prior to this study,it hasn′t been reported to obtain such high level productivity and 3HV fraction at the same time by Alcaligenes eutrophus.
【Fund】: 国家九五攻关项目(96 C0 3 0 3 0 2 )&&
【CateGory Index】: TQ320
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