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《Chinese Journal of Biotechnology》 2001-05
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Expression and Purification of Murine Interleukin 18 in Escherichia coli and Its Antitumor Effects

ZHOU Hu\ ZHAO Hui\|Ren *\ PEI Dong\|Sheng\ LU Liang\ Hu Shu\|Qun (Research Center for Biochemistry and Molecular Biology, Xuzhou Medical College, Xuzhou 221002, China)  
Total RNA was extracted from murine hepatocytes, and the cDNA of interleukin 18(IL\|18) was amplified by RT\|PCR.The cDNA was introduced into the expression vector pJW2 and sequenced. Under heat induction, the recombinant murine IL\|18(rmIL\|18) was expressed in inclusion bodies in E.coli with the yield accounting for 18% of total bacteria proteins. The inclusion bodies were dissolved with 5 mol/L urea, and rmIL\|18 was purified using Sephadex G\|100 column chromatography. In the presence of 0.5 mg/L Con A, the purified rmIL\|18 showed dose\|dependent IFN\|γ inducing activity in murine splenocytes. The purified rmIL\|18 exhibited significant antitumor effects in Kunming mice challenged intraperitoneally (i.p.) with H 22 hepatocarcinoma when administered 10μg rmIL\|18 i.p.on days 1,4 after challenge, and the mice survived resisted the rechallenged with H 22 cells.
【CateGory Index】: Q78
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