Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Biotechnology》 2001-05
Add to Favorite Get Latest Update

Expression and Purification of Murine Interleukin 18 in Escherichia coli and Its Antitumor Effects

ZHOU Hu\ ZHAO Hui\|Ren *\ PEI Dong\|Sheng\ LU Liang\ Hu Shu\|Qun (Research Center for Biochemistry and Molecular Biology, Xuzhou Medical College, Xuzhou 221002, China)  
Total RNA was extracted from murine hepatocytes, and the cDNA of interleukin 18(IL\|18) was amplified by RT\|PCR.The cDNA was introduced into the expression vector pJW2 and sequenced. Under heat induction, the recombinant murine IL\|18(rmIL\|18) was expressed in inclusion bodies in E.coli with the yield accounting for 18% of total bacteria proteins. The inclusion bodies were dissolved with 5 mol/L urea, and rmIL\|18 was purified using Sephadex G\|100 column chromatography. In the presence of 0.5 mg/L Con A, the purified rmIL\|18 showed dose\|dependent IFN\|γ inducing activity in murine splenocytes. The purified rmIL\|18 exhibited significant antitumor effects in Kunming mice challenged intraperitoneally (i.p.) with H 22 hepatocarcinoma when administered 10μg rmIL\|18 i.p.on days 1,4 after challenge, and the mice survived resisted the rechallenged with H 22 cells.
【CateGory Index】: Q78
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 ZHAO Chunyan,ZHAO Baochang ( Department of Biochemistry and Molecular Biology,Dalian Medical University,Dalian 116027 );Cloning and Expression of Human IL-18 Gene in E.coli[J];Chinese Journal of Biologicals;2003-01
【Citations】
Chinese Journal Full-text Database 3 Hits
1 Du Yong, Wang Tao, Du Guixin, Xu Jing, Hou Lihua, Wang Haitao (Department of Applied Molecular Biology, Beijing Institute of Microbiology and Epidemiology, Beijing 100071);CLONING OF PRECURSOR/ MATURE HUMAN INTERLEU KIN 18 GENES FROM HUMAN PBMC, EXPRESSION AND PURIFICATION OF THE PROTEINS IN E.coli[J];IMMUNOLOGICAL JOURNAL;1999-04
2 PEI Dong Sheng, HU Shu Qun, ZHAO Hui Ren * ( Research Center for Biochemistry and Molecular Biology, Xuzhou Medical College, Xuzhou 221002, China );Interleukin 18: High Level Expression in E.coli, Purification and Renaturation of the Recombinant Protein[J];ACTA BIOCHIMICA ET BIOPHYSICA SINICA;2000-04
3 HU Xiao *, WANG Lifeng, LU Ping, et al. *Department of Immunology, Beijing Medical University , Beijing 100083;Expression and analysis of recombinant human IL 18 and human IL 18 GFP fusion protein[J];CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY;1999-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 TIAN Wen biao, ZOU Quan ming, WU Chao, ZHANG Wei jun, YANG Jun, RAN Xiang yang, QANG Fu kun (Department of Clinical Microbiology, Third Military Medical University, Chongqing 400038, China);Purification and evaluation of the biological activity of recombinant E. coli heat-labile enterotoxin B[J];Acta Academiae Medicinae Militaris Tertiae;2003-02
2 ZHU Yong hong, ZOU Quan ming (Department of Clinical Microbiology and Immunology, Third Military Medical University, Chongqing 400038, China);Purification and characterization of non-fusion recombinant human interleukin-18 expressed in E. coli[J];Acta Academiae Medicinae Militaris Tertiae;2003-16
3 JIA Jing~1,LI hong~2,LI Ming-yuan~2,L Li-mei~1,YANG Zhi-hui~1,CHEN Wen-jie~1,JIA Yi~1,JIANG Zhong-hua~2,ZHANG Lin~1*(1.Key Laboratory of Biotherapy of Human Diseases, Ministry of Education, Sichuan University, Chengdu 610041,China;2.Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041,China);Construction of eukaryotic expression vector containing mouse IL-18 and examination of the bioactivity of the expressed IL-18[J];West China Journal of Pharmaceutical Sciences;2005-06
4 JIA Hong-Li 1,YANG Li-Min 2,WANG Jing-Lin 1*,KANG L in 1,WANG Li-Chun 2,SUN Si-Mei 1 (1. Institute of Microbiology and Epidemiology,Academy of Military Medical Sciences,Beijing 100071,China;2. Department of Biology,Inner Mongol ia University,Hohhot 010021,China);Expression, purification and antigenicity of recombinant C-terminal fragment of botulinum neurotoxin type E (BoNT/E) heavy chain[J];Bulletin of The Academy of Military(Medical Sciences);2004-05
5 TAO Jian-ping 1,LI Man-zhi 2,WANG Hui-min 2,GUO Xiang 3,HONG Ming-huang 3(1 Department of Microbiology,Sun Yat-Sen University of Medical Scienc e,Guangzhou 510080;2 Cancer Institute;3 Cancer Hospital);Study on the Tumorigenicity of Nasopharyngeal Carcinoma Cells Transfected with Human IL-18 cDNA in Hu-PBL-SCID Mice[J];Shanghai J.of Immunology;2002-06
6 ZHAO Jumin~(1,2), YANG Gengliang~(1,3), ZHENG Chao~1, LIU Haiyan~1, WANG Manman~1, CHEN Yi~3(1. School of (Pharmacy,) Hebei University, Baoding 071002, China;2. Quzhou Branch, Handan University, Handan 057250, China;3. Beijing Institute of Chemistry, The Chinese Academy of Sciences, Beijing 100080, China);Separation of Interleukin-18 on a Weak Anion Exchange Monolithic Column[J];Chinese Journal of Chromatography;2005-02
7 LU Zhong-Min, ZHAO Bao-Chang*(Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116027,China);Expression of Human Interleukin-18 Mutant Containing RGD Motif in Yeast and Its Inhibitory Effect on Platelet Aggregation[J];Chinese Journal of Biochemistry and Molecular Biology;2005-06
8 TENG Xia1, YANG Shen2, LI Qian1, SUN Man-ji2 1. Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850; 2. Shanghai Institute of Materia, Chinese Academy of Sciences, Shanghai 201203; China;Expression of Q-Type Human Paraoxonase in Escherichia.coli[J];Letters in Biotechnology;2007-01
9 GONG Xing-guo, YU Hong, XU Fei-hu, TAN Hao-si (Institute of Biologic Macromolecule and Enzymatic Engineering, College of Life Science, Zhejiang University, Hangzhou 310027, China);Construction and expression of fusion gene of LC-1 ScFv and GFP[J];Journal of Zhejiang University(Engineering Science);2005-05
10 NI Pei hua, WEN Wei ping, WANG Dan yi,et al. Department of Laboratory Medicine, Ruijing Hospital, Shanghai Second Medical University,200025, China;Application of Green Fluorescent Protein[J];Chinese Journal of Laboratory Diagnosis;2001-05
China Proceedings of conference Full-text Database 1 Hits
1 LU Min YU Hong GONG Xing-guo Institute of Biologic Macromolecule and Enzyme Engineering,College of Life Science,Zhejiang University,Hangzhou 310027;Cloning,Expression,Purification,and Functional Characterization of LC-1 ScFv with GFP tag[A];[C];2004
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 DONG Yuqing LI Ronggui\ DONG Chunxia\ ZHAO Jindong\ WU Guangyao (College of Life Sciences,Peking University,Beijing,100871);Identification and Expression of the Product from Engineering Bacterium Containing Recombinant Human Interleukin 18(IL-18) Gene[J];ACTA SCICENTIARUM NATURALUM UNIVERSITIS PEKINESIS;2000-02
2 CHEN Ji Quan 1, CAO Xue Tao 2, XIU Qing Yu 1, YU Yi Zhi 2, LUO Wen Tong 1 (1.Department of Respiratory Medicine, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China; 2. Department of Immunology, Department of Ba;Effect of intratracheal administration of recombinant adenovirus containing IL-18 gene in treatment of experimental lung metastasis[J];Academic Journal of Second Military Medical University;2000-06
3 SUN Qing, HUA Jun, HANG Xiaosheng, MAO Yongjie, WANG Qi. Department of Oncology, Wuxi No.2 People’s Hospital, Nanjing Medical University, Wuxi, Jiangsu 214001, P.R.China;Randomized clinical study comparing gemcitabine and oxaliplatin versus gemcitabine and cisplatin for advanced non-small cell lung cancer in elderly patients[J];Chinese Journal of Lung Cancer;2005-05
4 ZHANG Guo rong *,ZHAO Jian zeng,ZHANG Jin dong,WANG Xiao ping, CHEN Tao,ZHU Mei cai,LIU Ya ning,JIANG Shu qiang,WANG Zhe (Laboratory of Gene Therapy,Center for Clinical Molecular Biology,General Hospital of Air Force,Beijing 100036,China);Significant Antitumor Effects Mediated by Expression of the Precursor Form of Interleukin-18 (pro-IL-18) in Skeletal Muscle[J];Chinese Journal of Biochemistry and Molecular Biology;2001-01
5 GAO Luo yi, Robert K. YU, ZENG Gui chao * (Institution of Molecular Medicine and Genetics, Medical College of Georgia, 1120 15 th Street, CB-2803, Augusta, Georgia 30912, USA);Ganglioside GD3 and Tumor Angiogenesis[J];Chinese Journal of Biochemistry and Molecular Biology;2001-04
6 Li Wei, Ouyang Fan ( State Key Laboratory of Biochemical Engineering, institute of Chemical Metallurgy, Chinese Academy of Sciences, Beijing 100080, China );Comparasion of Three Renaturation Techniques of Recombinant Streptokinase[J];PHARMACEUTICAL BIOTECHNOLOGY;1999-04
7 HUANG Xiao, ZHOU Jue, ZHANG Feng kun,et al.(Department of Gynecology, Cancer Hospital,Fudan University, Shanghai 200032,China);Study on antitumor effect of IL-18 on ovarian carcinoma cell line HO-8910[J];China Oncology;2002-02
8 GUO Li An, ZHU Bao Quan a, CHEN Dai Jie a (The Centre Labaratory, 4th Military Medical University, Xi'an 710032; aShanghai Institute of Pharmaceutical Industry, Shanghai 200040);SELECTION OF REFOLDING CONDITIONS OF RECOMBINANT PROTEINS BY USING THE THREE STATE MODEL[J];CHINESE JOURNAL OF PHARMACEUTICALS;1999-03
9 HU Xiao *, WANG Lifeng, LU Ping, et al. *Department of Immunology, Beijing Medical University , Beijing 100083;Expression and analysis of recombinant human IL 18 and human IL 18 GFP fusion protein[J];CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY;1999-04
10 CHEN Jiquan *, CAO Xuetao, XIU Qingyu, et al. *Department of Respiratory Medicine, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;Treatment of spontaneous metastatic lung cancer with tumor antigen pulsed, interleukin 18 gene modified dendritic cells[J];National Medical Journal of China;2001-13
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 YANG Jing-sheng, YU Gang, HU Yong, et al (Wuhan Institute of Biological Products, Wuhan 430030, China);Mock Screening and Activity of Human Interleukin-18 Mutant[J];Chinese Journal of Biologicals;2008-08
2 JING Zhi-zhong, DOU Yong-xi, HOU Jun-lin, MENG Xue-lian, CAI Xue-peng(Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046,China);Cloning and sequence analysis of porcine interleukin-18 gene[J];Chinese Journal of Veterinary Science and Technology;2004-07
3 JING Zhi-zhong, DOU Yong-xi, LUO Qi-hui, CHEN Guo-hua, MENG Xue-lian, ZHENG Ya-dong, LUO Xue-nong, CAI Xue-peng ( Key Laboratory of Veterinary Parasitology of Gansu Province / State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046 );The Molecular Clone, Expression and Prediction of Construction to Key Genes of Interleukin Family of Porcine[J];Scientia Agricultura Sinica;2006-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved